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제품 설명
2×Hieff™ PCR Master Mix (With Dye)
제품 번호
10102ES03 / size: 1ml
10102ES08 / size: 5x1ml
10102ES50 / size: 50x1ml
제품 설명
2×HieffTM PCR Master Mix contains HieffTM Taq DNA Polymerase (Cat#10101), dNTPs, and other PCR-required components. The Master Mix is stable for 3 months at 4℃ with our customized stabilizers. The pre-mix solution is optimized for conventional PCR and ready to use by adding DNA template and primers. The PCR products can be loaded directly for electrophoresis with pre-loaded bromophenol blue dye. The amplified products contain 3 '-dA protrusion and can be easily cloned into T vector. The 2×HieffTM PCR Master Mix simplifies PCR procedure and reduces contamination.
Features
Applications
Fidelity (vs. Taq) | 1× |
Hot Start | No |
Overhang | 3 '-A |
Polymerase | Taq DNA Polymerase |
Reaction Format | SuperMix or Master Mix |
Reaction Speed | Standard |
Product Type | PCR Master Mix (2x) |
The 2×HieffTM PCR Master Mix products should be stored at -25~-15℃ for 2 years.
Figure 1 The expected 1.2 kb PCR products can be amplified with 2×HieffTM PCR Master Mix.
The Master Mix was stored at -20℃ for 1 year following another 3 months at 4℃ and 1 month at 25℃. Template: Arabidopsis genome. Annealing temperature: 60℃. Extension time: 40 sec.
Cited from "Ultrasensitive sensors reveal the spatiotemporal landscape of lactate metabolism in physiology and disease" Cell Metab . 2023 Jan 3;35(1):200-211.e9. doi: 10.1016/j.cmet.2022.10.002
Ver.EN20230901
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