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YEASEN

[YEASEN] Hieff™ Bst Plus DNA Polymerase

Cat-No. 14402ES

YEASEN은 생명과학 연구 및 진단을 위한 고품질의 연구용 시약과 기기를 제공하는 글로벌 바이오 기업입니다.

제품 설명

Hieff™ Bst Plus DNA Polymerase

제품 번호

14402ES92 / size: 8000U

14402ES97 / size: 40000U

제품 설명

Description

Hieff™ Bst Plus DNA Polymerase is derived from Thermophilic Geobacillus sp DNA Polymerase I, lacking 5'-3' exonuclease activity. The enzyme has stronger 5'-3' DNA polymerase activity, strand displacement activity, and dUTP tolerance, which is more suitable for anti-pollution isothermal amplification reactions, such as LAMP, CPA, etc.

Features

Thermophilic DNA polymerase with strong strand displacement activity

Applications

Isothermal DNA amplification

Specifications

Polymerase	Bst DNA Polymerase
Heat Inactivation	Incubation at 85°C for 5 min.
Solution Components	10 mmol/L Tris-HCl, 50 mmol/L KCl, 0.1 mmol/L EDTA, 1 mmol/L DTT, 0.1% Triton X-100, 50% Glycerol, pH 7.5 @ 25℃.
Activity Definition	1 U refers to the amount of enzyme required to incorporate 10 nmol of dNTP into the acid-insoluble precipitate in 30 min at 65°C.

Components

Components No.	Name	14402ES92 (8,000 U)	14402ES97 (40,000 U)
14402-A	Hieff™ Bst Plus DNA Polymerase (40 U/μL)	200 μL	1 mL
14402-B	10 ×Hieff™ Bst Plus DNA Polymerase Buffer	1 mL	3×1 mL
14402-C	100 mmol/L MgSO4	1 mL	3×1 mL

Figures

High Sensitivity

Figure 1: Sensitivity Test

Red: 50 copies/T, response less than 15 minutes
Figure 1: The copy numbers of 50 copies/Tweres tested. The test results showed that within 15 minutes, the results of 50 copies/T performed better, and the detection rate reached 100%.

Stability

Figure 2.Stability testing at the same temperature

Red: 37℃ 0 day Blue: 37℃ 1 day Green: 37℃ 3 days Purple: 37℃ 5 days
0 days at 37°C as a control and stability tests were carried out for 1, 3, and 5 days at the same temperature, indicating that it can still maintain a relatively stable effect at 37°C for 5 days.