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YEASEN

[YEASEN] Hieff Trans™ Liposomal Transfection Reagent

Cat-No. 40802ES

40802ES02 / size: 0.5ml
40802ES03 / size: 1ml
40802ES08 / size: 5x1ml

YEASEN은 생명과학 연구 및 진단을 위한 고품질의 연구용 시약과 기기를 제공하는 글로벌 바이오 기업입니다.

제품 설명

Hieff Trans™ Liposomal Transfection Reagent

제품 번호

40802ES02 / size: 0.5ml

40802ES03 / size: 1ml

40802ES08 / size: 5x1ml

제품 설명

Description

Hieff Trans™ Liposomal Transfection Reagent is a versatile liposome transfection reagent, suitable for DNA, RNA, and oligonucleotide transfection, with high transfection efficiency for most eukaryotic cells. Its unique formula allows it to be added directly to the medium, and the presence of serum does not affect transfection efficiency, which reduces the damage to cells caused by serum removal. There is no need to remove the nucleic acid-Hieff Trans™ complex or replace it with a fresh medium after transfection, and it can also be removed after 4-6 hours.

Hieff Trans™ is supplied in sterile liquid form. Usually, for 24-well plate transfection, about 1.5 μL each time, 1 mL of Hieff Trans™ can do about 660 transfections; for a 6-well plate, about 6 μL each time, 1 mL of Hieff Trans™ can do about 660 transfections. 160 transfections.

Feature

Exceptional Efficiency: Superior transfection performance in the broad range of cell lines, including transient transfection and stable transfection
Wide Adaptability: Excellent transfection efficiency in a variety of cell lines and high levels of recombinant protein expression
Low Toxicity: The activities of the diverse cells almost unaffected by transfection reagents from YEASEN
Simple Operation: Proven efficacy in the presence of serum—eliminates the need to change media following transfection
Cost Effective: Competitive transfection effect with more affordable prices

Application

Cell Transfection

Specification

Form	Liquid
Serum Compatible	Yes
Cell Type	Established Cell Lines
Sample Type	Plasmid DNA, Synthetic siRNA
Transfection Technique	Lipid-Based Transfection

Components

Components No.	Name	40802ES02	40802ES03	40802ES08
40802	Hieff TransTM;Liposomal Transfection Reagent	0.5 mL	1 mL	5×1 mL

Shipping and Storage

The product is shipped with ice packs and can be stored at 2-8ºC for one year. Do not freeze!

Figures

Demonstration of Transfection Effect

Figure 1. Hieff Trans™ Liposome Transfection Reagent outperforms the transfection reagent from the competitive brand（T* brand in Figure 1a, P* brand in Figure 1b).

Each reagent was used to transfect the target cell line（Hela cell line in Figure 1a, DF-1 cell line in Figure 1b）in a 96-well format. GFP expression was analyzed 48 hours posttransfection. Hieff Trans™ Liposome Transfection Reagent provided higher GFP transfection efficiency than the competitive brand products.

Validated Cell Lines

Product Name	Hieff TransTMLiposomal Transfection Reagent
Validated Cell Lines	293T	Calu 1	HEK293	HO1980	N2A	TS
	293F	CHO-K1	HEK293T	HUVEC	NCI-H1975	U-87
	293FT	COS-7	Hela	MCF10A	NIH-3T3	Vero
	3T3	DF-1	Hep2C	MCF-7	Neuro-2a	WEHI
	A549	H1299	Hep3B	MDA-MB-231	PC-12	WRL-68
	BV-2	H520	Hepa1-6	MDA-MB-231-LM2-4175	Raw264.7
	B50	HaCaT	HepG2	MDCK	SGC-7901
	C2C12	HCT116	HK2	MEF	T47D	…

Figure 2. List of cell lines successfully transfected with Hieff Trans™ Liposomal Transfection Reagent (Under continuous update).

Cited from "Optogenetic control of RNA function and metabolism using engineered light-switchable RNA-binding proteins. Nat Biotechnol. 2022 Jan 3. doi: 10.1038/s41587-021-01112-1"

Cited from "Structural insight into BRCA1-BARD1 complex recruitment to damaged chromatin. Mol Cell. 2021 Jul 1;81(13):2765-2777.e6. doi: 10.1016/j.molcel.2021.05.010."

Cited from "UBQLN2-HSP70 axis reduces poly-Gly-Ala aggregates and alleviates behavioral defects in the C9ORF72 animal model. Neuron. 2021 Jun 16;109(12):1949-1962.e6. doi: 10.1016/j.neuron.2021.04.023."

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