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YEASEN

[YEASEN] D-Luciferin, Potassium Salt

Cat-No. 40902ES
40902ES01 / size: 100mg
40902ES02 / size: 500mg
40902ES03 / size: 1g

YEASEN은 생명과학 연구 및 진단을 위한 고품질의 연구용 시약과 기기를 제공하는 글로벌 바이오 기업입니다.




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D-Luciferin, Potassium Salt




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40902ES01 / size: 100mg

40902ES02 / size: 500mg

40902ES03 / size: 1g

 



제품 설명


Description

D-luciferin is a common substrate for Luciferase and is widely used throughout biotechnology, especially in vivo imaging Technology. The mechanism of action is that luciferin (substrate) can be oxidized to emit light in the presence of ATP and luciferase. When fluorescein is in excess, the quantum number of light produced is equal to Luciferase concentrations were positively correlated (see figure below). Plasmids carrying luciferase encoding gene (Luc) were transfected into cells and introduced into study animals such as rats and mice.  

D-luciferin is also commonly used in in vitro studies, including luciferase and ATP levels analysis; Reporter gene analysis; High-throughput sequencing and various contamination tests. There are three Product form: D-luciferin (free acid), D-luciferin salt (sodium salt and potassium salt). The main difference lies in solubility: water solubility of the former and solubility of the buffer system. They are weakly soluble except in weak bases such as low concentrations of NaOH and KOH solutions. Soluble in methanol and DMSO; The latter can be easily dissolved in water or buffer, easy to use.

Features

  • No radiation, almost harmless to living organisms.
  • Bioluminescence, no excitation light source.
  • So sensitive, you can detect it in a few hundred cells.
  • Good penetration, 3-4cm tissue depth can still be detected.
  • High signal-to-noise ratio, strong fluorescence signal and good anti-interference.

Applications

  • In the tumorigenesis experiment in nude mice, the tumor growth was observed without invasion in real time, without tumor stripping measurement.
  • To test the effect of the administration on tumor growth or metastasis, the fluorescein substrate can be completely eliminated within 3 hours, without interference to the drug experiment.
  • The localization and distribution of foreign cells in animals were detected.
  • The target gene or promoter of the target gene is fused to the luciferase gene to detect changes in gene expression during drug treatment or disease progression.
  • Monitoring stem cell transplantation, survival and proliferation; Trace the distribution and migration of stem cells in vivo.

Specifications

English synonym(S)-4,5-Dihydro-2-(6-hydroxy-2-benzothiazolyl)-4-thiazolecarboxylic acid potassium salt; D-Luciferin firefly, potassium salt
CAS NO.115144-35-9
FormulaC11H7N2O3S2K
Molecular weight318.42 g/mol
AppearanceLight yellow powder
SolubilitySoluble in water(60 mg/mL)


Components

Components No.Name40902ES0140902ES0240902ES0340902ES08
40902D-Luciferin, Potassium Salt100mg500 mg1g5g


Shipping and Storage

The product is shipped with dry ice and can be stored at -15℃ ~ -25℃ for 1 year.


Citations & References:

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[18] Zhang X, Li Y, Ji J, et al. Gadd45g initiates embryonic stem cell differentiation and inhibits breast cell carcinogenesis. Cell Death Discov. 2021;7(1):271. Published 2021 Oct 2. doi:10.1038/s41420-021-00667-x(IF:5.241)

[19] Wu D, Lv J, Zhao R, et al. PSCA is a target of chimeric antigen receptor T cells in gastric cancer. Biomark Res. 2020;8:3. Published 2020 Jan 28. doi:10.1186/s40364-020-0183-x(IF:4.866)

[20] Qiao K, Chen C, Liu H, Qin Y, Liu H. Pinin Induces Epithelial-to-Mesenchymal Transition in Hepatocellular Carcinoma by Regulating m6A Modification. J Oncol. 2021;2021:7529164. Published 2021 Dec 7. doi:10.1155/2021/7529164(IF:4.375)

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