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[YEASEN] G418 Sulfate (Geneticin)

Cat-No. 60220ES

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제품 설명

G418 Sulfate (Geneticin)

제품 번호

60220ES03 - 1g

60220ES08 - 5g

제품 설명

Description

G418 Sulfate, also known as Geneticin Sulfate, is an amino-glycoside antibiotic and structurally similar to Gentamycin B1. It interfers with protein synthesis by binding 80s ribosome and blocking elongation steps. G418 Sulfate is toxic to both prokaryotic and eukaryotic cells, including bacteria, yeast, higher plant and mammalian cells, as well as protozoans and worms. The resistance gene (mainly neo), locating in transposon Tn601 (903) or Tn5, is derived from bacteria, but can be expressed in eukaryotic cells. The resistance gene can be introduced into cells through gene recombination techniques to obtain resistance of G418, which could be used to screen and maintain the culture of prokaryotic or eukaryotic cells carrying resistance gene.
In mammalian cells, neo,encodes the expression of amino-glycoside 3' -phosphotransferase (APH (3') II) after integrated into the eukaryotic genome. This enzyme inactivates the antibiotic by covalently modifying the amino or hydroxyl function of G418 and inhibiting the antibiotic-ribosome interaction, endowing the cell with G418 resistance. In screening stable cell line experiments, the killing curves (dose-response curves) should be established to determine the minimum effective concentration for killing non-resistant cells.
In plant cells, resistance can be obtained by transfection of nptII gene resistant plasmid. The nptII gene also encodes aminoglycoside phosphotransferase, an enzyme that inactivates several antibiotics, including G418, kanamycin, and palomycin.

Features

High-quality raw materials, all antibiotic product raw materials are sourced from high-quality fixed suppliers
Standardized production, using factory mass production mode
Wide range of applications, which can be used in the fields of molecular biology and biochemical experimental research of tissue culture
The cooperation platform covers the whole
To ensure product quality stability, the difference between batches is controlled within 1%

Applications

Screening of stably transfected cell lines

Specifications

Synonym	G-418 disulfate; Geneticin sulfate; Antibiotic G418; G 418 Sulfate; Antibiotic G-418 sulfate
CAS No.	108321-42-2
Molecular formula	C₂₀H₄₀N₄O₁₀·2H₂SO₄
Molecular weight	692.7 g/mol
Appearance	White or light white powder
Purity	≥98%
Potency（anhydrous）	> 700 U/mg
Solubility	≥60 mg/mL in H2O; insoluble in EtOH; insoluble in DMSO
Structure

Components

Components No.	Name	60224ES03	60224ES08
60220	G418 Sulfate (Geneticin)	1 g	5 g

Storage

The product should be stored at -25℃ ~ -15℃ for 3 years. Avoid moisture, which can reduce antibiotic activity.

Figures

Figure 1 Stability test and validation of effective concentration of genomycin between different batches

Figure 1 Stability test and validation of effective concentration of genomycin between different batches

Experimental strain: E. coli
G69 and G99 were two different batches;
The use concentration of genomycin was determined to be 8 mg/L, 12 mg/L and 16 mg/L respectively, and the effective minimum concentration was determined to be 16 mg/L, which perfectly inhibited the growth of miscellaneous bacteria. The performance of the two batches was consistent.

Citations & References:

[1] Zhu G, Xie J, Kong W, et al. Phase Separation of Disease-Associated SHP2 Mutants Underlies MAPK Hyperactivation. Cell. 2020;183(2):490-502.e18. doi:10.1016/j.cell.2020.09.002(IF:38.637)

[2] Zhang D, Liu Y, Zhu Y, et al. A non-canonical cGAS-STING-PERK pathway facilitates the translational program critical for senescence and organ fibrosis. Nat Cell Biol. 2022;24(5):766-782. doi:10.1038/s41556-022-00894-z(IF:28.824)

[3] Zhou C, Yi C, Yi Y, et al. LncRNA PVT1 promotes gemcitabine resistance of pancreatic cancer via activating Wnt/β-catenin and autophagy pathway through modulating the miR-619-5p/Pygo2 and miR-619-5p/ATG14 axes. Mol Cancer. 2020;19(1):118. Published 2020 Jul 29. doi:10.1186/s12943-020-01237-y(IF:27.401)

[4] Meng F, Yu Z, Zhang D, et al. Induced phase separation of mutant NF2 imprisons the cGAS-STING machinery to abrogate antitumor immunity. Mol Cell. 2021;81(20):4147-4164.e7. doi:10.1016/j.molcel.2021.07.040(IF:17.970)

[5] Zhou C, Liang Y, Zhou L, et al. TSPAN1 promotes autophagy flux and mediates cooperation between WNT-CTNNB1 signaling and autophagy via the MIR454-FAM83A-TSPAN1 axis in pancreatic cancer. Autophagy. 2021;17(10):3175-3195. doi:10.1080/15548627.2020.1826689(IF:16.016)

[6] Chen S, Liu S, Wang J, et al. TBK1-Mediated DRP1 Targeting Confers Nucleic Acid Sensing to Reprogram Mitochondrial Dynamics and Physiology. Mol Cell. 2020;80(5):810-827.e7. doi:10.1016/j.molcel.2020.10.018(IF:15.584)

[7] Zhao Q, Zheng K, Ma C, et al. PTPS Facilitates Compartmentalized LTBP1 S-Nitrosylation and Promotes Tumor Growth under Hypoxia. Mol Cell. 2020;77(1):95-107.e5. doi:10.1016/j.molcel.2019.09.018(IF:14.548)

[8] Yu P, Zhu X, Zhu JL, et al. The Chk2-PKM2 axis promotes metabolic control of vasculogenic mimicry formation in p53-mutated triple-negative breast cancer. Oncogene. 2021;40(34):5262-5274. doi:10.1038/s41388-021-01933-z(IF:9.867)

[9] Wu Y, Gu W, Han X, Jin Z. LncRNA PVT1 promotes the progression of ovarian cancer by activating TGF-β pathway via miR-148a-3p/AGO1 axis. J Cell Mol Med. 2021;25(17):8229-8243. doi:10.1111/jcmm.16700(IF:5.310)

[10] Zeng WJ, Lu C, Shi Y, et al. Initiation of stress granule assembly by rapid clustering of IGF2BP proteins upon osmotic shock. Biochim Biophys Acta Mol Cell Res. 2020;1867(10):118795. doi:10.1016/j.bbamcr.2020.118795(IF:4.105)

[11] Li B, Zhang J, Su Y, et al. Overexpression of PTEN may increase the effect of pemetrexed on A549 cells via inhibition of the PI3K/AKT/mTOR pathway and carbohydrate metabolism. Mol Med Rep. 2019;20(4):3793-3801. doi:10.3892/mmr.2019.10617(IF:1.851)

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