(주)모아바이오

YEASEN

[YEASEN] Hygromycin B

Cat-No. 60225ES

YEASEN은 생명과학 연구 및 진단을 위한 고품질의 연구용 시약과 기기를 제공하는 글로벌 바이오 기업입니다.

제품 설명

Hygromycin B

제품 번호

60225ES03 - 1g

60225ES10 - 10g

제품 설명

Description

Hygromycin B, an aminoglycoside antibiotic synthesized by Streptomyces hygroscopicus, inhibits protein synthesis by interfering with 70S ribosomal translocation and inducing misreading of mRNA template, thus killing prokaryotes (such as bacteria), eukaryotes (such as yeasts, fungi) and higher mammalian eukaryotes.

Hygromycin resistance genes (hyg or hph) derived from Escherichia coli encode hygromycin B phosphotransferase, which detoxifies hygromycin B into a non-biologically active phosphorylated product, making it an excellent selective marker for screening and culturing prokaryotic or eukaryotic cells successfully transfected with hytromycin resistance genes. In addition, due to different modes of action, Hygromycin B is often used in combination with G418 or Blasticidin S for the selection of dual-resistant cell lines. Hygromycin B can also be used as an antiviral agent because it selectively penetrates into cells with increased membrane permeability due to viral infection and inhibits translation. It can also act as an insect-repellant via mixed with animal feed.

Features

High-quality raw materials, all antibiotic product raw materials are sourced from high-quality fixed suppliers
Standardized production, using factory mass production mode
Wide range of applications, which can be used in the fields of molecular biology and biochemical experimental research of tissue culture
The cooperation platform covers the whole
To ensure product quality stability, the difference between batches is controlled within 1%

Applications

Screening of stably transfected cell lines

Specifications

Cat.No.	60225ES03 / 60225ES10
Size	1 g/10 g
CAS No.	31282-04-9
Molecular formula	C₂₀H₃₇N₃O₁₃
Molecular weight	527.52 g/mol
Purity	>90% (HPLC)
Potency	≥1050 U/mg
Structure

Components

Name	60225ES03	60225ES10
Size	1 g	10 g

Storage

The product should be stored at -25～-15℃ for two years.

Figures

Figure 1 Colony growth of E. coli on hygromycin resistant plates with different concentrations, YEASEN and brand S* have the same effect

Experimental strain: E. coli
Note: A-C are brand S* hygromycin 20 μg/ml, 50 μg/ml, 100 μg/ml plates, D-F are Yeasen hygromycin 20 μg/ml, 50 μg/ml, 100 μg/ml plates

Citations & References:

[1] Zhao Q, Zheng K, Ma C, et al. PTPS Facilitates Compartmentalized LTBP1 S-Nitrosylation and Promotes Tumor Growth under Hypoxia. Mol Cell. 2020;77(1):95-107.e5. doi:10.1016/j.molcel.2019.09.018(IF:14.548)

[2] Zhu GD, Yu J, Sun ZY, et al. Genome-wide CRISPR/Cas9 screening identifies CARHSP1 responsible for radiation resistance in glioblastoma. Cell Death Dis. 2021;12(8):724. Published 2021 Jul 21. doi:10.1038/s41419-021-04000-3(IF:8.469)

[3] Chen J, Huang Y, Tang Z, et al. Genome-Scale CRISPR-Cas9 Transcriptional Activation Screening in Metformin Resistance Related Gene of Prostate Cancer. Front Cell Dev Biol. 2021;8:616332. Published 2021 Jan 26. doi:10.3389/fcell.2020.616332(IF:6.684)

[4] Jiang Z, Cui Z, Zhu Z, et al. Engineering of Yarrowia lipolytica transporters for high-efficient production of biobased succinic acid from glucose. Biotechnol Biofuels. 2021;14(1):145. Published 2021 Jun 27. doi:10.1186/s13068-021-01996-w(IF:6.040)

[5] Liu Y, Jiang X, Cui Z, Wang Z, Qi Q, Hou J. Engineering the oleaginous yeast Yarrowia lipolytica for production of α-farnesene. Biotechnol Biofuels. 2019;12:296. Published 2019 Dec 23. doi:10.1186/s13068-019-1636-z(IF:5.452)

[6] Cui Z, Zheng H, Jiang Z, et al. Identification and Characterization of the Mitochondrial Replication Origin for Stable and Episomal Expression in Yarrowia lipolytica. ACS Synth Biol. 2021;10(4):826-835. doi:10.1021/acssynbio.0c00619(IF:5.110)

YEASEN의 모든 제품을 만나 보세요!

Biopharma

mRNA enzyme

Nucleotides

Viral Vector Manufacturing

Quality Analysis Kit

NGS Library Preparation

Adapter

DNA Library Preparation

RNA Library Preparation

NGS Module

NGS enzyme