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제품 설명
Anti-CD147/BSG Polyclonal Antibody
제품 번호
PHE14001
제품 특징
Catalog No.
PHE14001
Species reactivity
Human
Host species
Rabbit
Isotype
IgG
Clonality
Polyclonal
Immunogen
E. coli - derived recombinant Human CD147/BSG (Gly140-Ala323).
Tested applications
ELISA: 1:4000-1:8000, IHC: 1:50-1:100, WB: 1:1000-1:4000
Target
Hepatoma-associated antigen,BSG,HAb18G,5F7,TCSF,OK blood group antigen,Collagenase stimulatory factor,CD147,Leukocyte activation antigen M6,Basigin,Extracellular matrix metalloproteinase inducer,EMMPRIN,Tumor cell-derived collagenase stimulatory factor
Purification
Purified by antigen affinity column.
Accession
P35613
Applications
ELISA, IHC, WB
Form
Liquid
Storage buffer
0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Data Image
Various lysates were subjected to SDS PAGE followed by western blot with CD147 / BSG antibody (PHE14001) at 0.48μg/ml.
Lane 1: 293T cell lysate
Lane 2: A2780 cell lysate
Lane 3: U937 cell lysate
Second Ab: Goat Anti-Rabbit IgG H&L Polyclonal antibody, HRP (PTB96431) at 0.1 μg/mL.
Predict MW: 42 kDa
Observed MW: 58 kDa
Immunocytochemistry/ Immunofluorescence
CD147 / BSG in HepG2 Cell Line.
The HepG2 cells were fixed with 4% paraformaldehyde (20 min), and then blocked with 5% goat serum for 1h. And the cells were incubated for 2h at 37°C with CD147 / BSG (PHE14001) at 9.6 μg/ml. The section was then incubated with Goat Anti-Rabbit IgG (Alexa Fluor-488) preabsorbed at 1/100 dilution (Shown in green) for 1 hour at room temperature.Nuclear DNA was labelled with DAPI (shown in blue).
Recombinant Protein lysates were subjected to SDS PAGE followed by western blot with CD147/BSG antibody (PHE14001) at 1 µg/ml.
Lane 1: Recombinant Protein
Second Ab: Goat Anti-Rabbit IgG H&L Polyclonal antibody, HRP (PTB96431) at 0.1 μg/mL.
Predict MW: 22 kDa
Observed MW: 22 kDa
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