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Antibody System

[Antibodysystem] Anti-CD147/BSG Polyclonal Antibody

Cat-No. PHE14001

AntibodySystem는 Primary Antibody Biosimilar - Research Grade

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제품 설명

Anti-CD147/BSG Polyclonal Antibody

제품 번호

PHE14001

제품 특징

Catalog No.

PHE14001

Species reactivity

Human

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human CD147/BSG (Gly140-Ala323).

Tested applications

ELISA: 1:4000-1:8000, IHC: 1:50-1:100, WB: 1:1000-1:4000

Target

Hepatoma-associated antigen,BSG,HAb18G,5F7,TCSF,OK blood group antigen,Collagenase stimulatory factor,CD147,Leukocyte activation antigen M6,Basigin,Extracellular matrix metalloproteinase inducer,EMMPRIN,Tumor cell-derived collagenase stimulatory factor

Purification

Purified by antigen affinity column.

Accession

P35613

Applications

ELISA, IHC, WB

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Data Image

Western blot
Various lysates were subjected to SDS PAGE followed by western blot with CD147 / BSG antibody (PHE14001) at 0.48μg/ml.

Lane 1: 293T cell lysate
Lane 2: A2780 cell lysate
Lane 3: U937 cell lysate

Second Ab: Goat Anti-Rabbit IgG H&L Polyclonal antibody, HRP (PTB96431) at 0.1 μg/mL.

Predict MW: 42 kDa
Observed MW: 58 kDa
Immunocytochemistry/ Immunofluorescence
CD147 / BSG in HepG2 Cell Line.
The HepG2 cells were fixed with 4% paraformaldehyde (20 min), and then blocked with 5% goat serum for 1h. And the cells were incubated for 2h at 37°C with CD147 / BSG (PHE14001) at 9.6 μg/ml. The section was then incubated with Goat Anti-Rabbit IgG (Alexa Fluor-488) preabsorbed at 1/100 dilution (Shown in green) for 1 hour at room temperature.Nuclear DNA was labelled with DAPI (shown in blue).
Western Blot
Recombinant Protein lysates were subjected to SDS PAGE followed by western blot with CD147/BSG antibody (PHE14001) at 1 µg/ml.

Lane 1: Recombinant Protein

Second Ab: Goat Anti-Rabbit IgG H&L Polyclonal antibody, HRP (PTB96431) at 0.1 μg/mL.

Predict MW: 22 kDa
Observed MW: 22 kDa