본문 바로가기 주메뉴 바로가기

제품소개

제품상세페이지

Antibody System

[Antibodysystem] Anti-CD147/BSG Polyclonal Antibody

Cat-No. PHE14001



AntibodySystem는 Primary Antibody Biosimilar - Research Grade 

제품을 전문적으로 생산하는 20년의 경험이 있는 High Quality 제조사입니다. 





제품 설명 


Anti-CD147/BSG Polyclonal Antibody

 



제품 번호


PHE14001




제품 특징

Catalog No.

PHE14001

Species reactivity

Human

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human CD147/BSG (Gly140-Ala323).

Tested applications

ELISA: 1:4000-1:8000, IHC: 1:50-1:100, WB: 1:1000-1:4000

Target

Hepatoma-associated antigen,BSG,HAb18G,5F7,TCSF,OK blood group antigen,Collagenase stimulatory factor,CD147,Leukocyte activation antigen M6,Basigin,Extracellular matrix metalloproteinase inducer,EMMPRIN,Tumor cell-derived collagenase stimulatory factor

Purification

Purified by antigen affinity column.

Accession

P35613

Applications

ELISA, IHC, WB

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.




Data Image


  • Western blot
    Various lysates were subjected to SDS PAGE followed by western blot with CD147 / BSG antibody (PHE14001) at 0.48μg/ml.

    Lane 1: 293T cell lysate
    Lane 2: A2780 cell lysate
    Lane 3: U937 cell lysate

    Second Ab: Goat Anti-Rabbit IgG H&L Polyclonal antibody, HRP (PTB96431) at 0.1 μg/mL.

    Predict MW: 42 kDa
    Observed MW: 58 kDa
  • Immunocytochemistry/ Immunofluorescence
    CD147 / BSG in HepG2 Cell Line.
    The HepG2 cells were fixed with 4% paraformaldehyde (20 min), and then blocked with 5% goat serum for 1h. And the cells were incubated for 2h at 37°C with CD147 / BSG (PHE14001) at 9.6 μg/ml. The section was then incubated with Goat Anti-Rabbit IgG (Alexa Fluor-488) preabsorbed at 1/100 dilution (Shown in green) for 1 hour at room temperature.Nuclear DNA was labelled with DAPI (shown in blue).
  • Western Blot
    Recombinant Protein lysates were subjected to SDS PAGE followed by western blot with CD147/BSG antibody (PHE14001) at 1 µg/ml.

    Lane 1: Recombinant Protein

    Second Ab: Goat Anti-Rabbit IgG H&L Polyclonal antibody, HRP (PTB96431) at 0.1 μg/mL.

    Predict MW: 22 kDa
    Observed MW: 22 kDa




AntibodySystem의 모든 제품들을 만나 보세요!  


Products

Recombinant Protein

Antibody

Assay Kit

Research Biosimilar

Enzyme

Trending Product

Super-Affinity Antibody

Cell Lysates



Antibodysystem - Official Distributor in South Korea "Morebio" 한국 독점 대리점 "모아바이오"