Paraoxonase-1 Isoform Human Recombinant is expressed in E. coli, fused to a 7 amino acid c- terminal hexahistidine tag, containing 362 amino acids and ( 1-355 a.a.) having a total molecular weight of 40.68kDa.

The PON1 purified by proprietary chromatographic techniques.

White lyophilized (freeze-dried) powder.

lyophilized from 0.5 mg/mL in 20mM Tris buffer, 50 mM NaCl, pH 7.5

Store at 4°C if entire vial will be used within 1-2 weeks. Store, frozen at -20°C for longer periods of time.

Avoid multiple freeze-thaw cycles.

It is recommended to reconstitute the lyophilized PON1 in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

MAKLIALTLLGMGLALFRNHQSSYQTRLNALREVQPVELPNCNLVKGIETGSEDLEILPNGL
AFISSGLKYPGIKSFNPNSPGKILLMDLNEEDPTVLELGITGSKFDVSSFNPHGISTFTDEDNA
MYLLVVNHPDAKSTVELFKFQEEEKSLLHLKTIRHKLLPNLNDIVAVGPEHFYGTNDHYFLD
PYLQSWEMYLGLAWSYVVYYSPSEVRVVAEGFDFANGINISPDGKYVYIAELLAHKIHVYEK
HANWTLTPLKSLDFNTLVDNISVDPETGDLWVGCHPNGMKIFFYDSENPPASEVLRIQNILT
EEPKVTQVYAENGTVLQGSTVASVYKGKLLIGTVFHKALYCELEHHHHHH.

Greater than 90% as determined by SDS-PAGE.

SDS