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제품 설명
Elab Fluor® Red 780 Anti-Mouse CD45 Antibody[30-F11]
제품 번호
E-AB-F1136US
제품 특징
Antigen Details
| Alternate Names | Receptor-type tyrosine-protein phosphatase C,Ptprc,L-CA,Ly-5,T200,CD45 |
| Swissprot | P06800 |
| References | 1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press. 2. Trowbridge IS, et al. 1993. Annu. Rev. Immunol. 12:85. 3. Kishihara K, et al. 1993. Cell 74:143. 4. Pulido R, et al. 1988. J. Immunol. 140:3851. |
| Form | Liquid |
| Clone No. | 30-F11 |
| Host | Rat |
| Isotype | Rat IgG2b, κ |
| Isotype Control | Elab Fluor® Red 780 Rat IgG2b, κ Isotype Control[LTF-2] [Product E-AB-F09843S] |
| Reactivity | Mouse |
| Application | FCM |
| Storage Buffer | Phosphate buffered solution, pH 7.2, containing 0.09% stabilizer and 1% protein protectant. |
| Recommended Use | Each lot of this antibody is quality control tested by flow cytometric analysis. Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use. We suggest each investigator should titrate the reagent to obtain optimal results [The recommended concentration is 0.1-1 μg/106 cells in 100 μL volume]. |
| Shipping | Biological ice pack at 4℃ |
| Stability & Storage | Keep as concentrated solution. Store at 2~8°C and protected from prolonged exposure to light. Do not freeze. Centrifuge before opening to ensure complete recovery of vial contents. This product is guaranteed up to one year from purchase. |
| Conjugation | Elab Fluor® Red 780 |
| Elab Fluor® Red 780 is designed to be excited by the Red (627-640 nm) laser and detected using an optical filter centered near 770 nm (e.g., a 780/60 nm bandpass filter). | |
| |
How to select the appropriate detection channel through the spectrogram?
| CD45 is a 180-240 kD glycoprotein also known as the leukocyte common antigen (LCA), T200, or Ly-5. It is a member of the protein tyrosine phosphatase (PTP) family, expressed on all hematopoietic cells except mature erythrocytes and platelets. There are different isoforms of CD45 that arise from alternative splicing of exons 4, 5, and 6, which encode A, B, and C determinants, respectively. CD45 plays a key role in TCR and BCR signal transduction. These isoforms are very specific to the activation and maturation state of the cell as well as cell type. The primary ligands for CD45 are galectin-1, CD2, CD3, CD4, TCR, CD22, and Thy-1. |
| Related Protocols | Staining Cell Surface Targets for Flow Cytometry |
| Troubleshooting |
DOI: 10.1155/2023/2894695 | |
| Sample: Colonic Lamina Propria Cell |
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