DSMZ no.:

Species:

human (Homo sapiens)

Cell type:

Burkitt lymphoma

Origin:

established in 1973 from the inguinal tumor of an EBV-negative 5-year-old girl with Burkitt lymphoma at the preterminal stage; although the tumor tissue was experimentally exposed to EBV, neither EBV sequences nor EBV antigens were detectable in the established cell line. Exome and RNA sequence data are available (see Ref 18187 and Exome sequence and RNA-Seq)

Reference(s):

18899, 15230, 18187

Biosafety level:

1

Permissions and restrictions:

Morphology:

round to polymorphic cells growing in suspension singly cells and in clumps; image; image

Medium:

80% RPMI 1640 + 20% h.i. FBS (+10% FBS after one week)

Subculture:

seed out at ca. 0.5-0.7 x 10⁶ cells/ml; optimal split ratio 1:2 -1:5 every 2-3 days; culture flask should be kept horizontal

Incubation:

at 37 °C with 5% CO₂

Doubling time:

ca. 30-40 hours

Harvest:

maximum density at ca. 1.5 x 10⁶ cells/ml

Storage:

frozen with 70% medium, 20% FBS, 10% DMSO

Mycoplasma:

negative in PCR assay

Immunology:

CD3-, CD10+, CD13-, CD19+, CD20+, CD34-, CD37+, CD38+, CD80-, CD138(+), HLA-DR+, cyCD79a+, cIgG-, cIgM+, cIg-kappa+, cIg-lambda-, sIgG-, sIgM+, sIg-kappa+, sIg-lambda-; image

Authenticity:

STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.

Cytogenetics:

human hypodiploid karyotype; 44(40-46)<2n>XX, -10, -21, der(6)t(6;10)(p22;p12), der(7)t(1;7)(p36;p22), t(11;17)(q24;q24), der(11)t(11;17)(q24;q24)t(11;6)(p15;q11)t(6;5)(q22;?), ider(13)(q10)t(3;13)(q26;q14), der(13)t(10;13)(q11;p11)dup(13)(q11q32)t(13;21)(q32;q22), der(21)t(13;21); does not correspond with previous BJA-B karyotypes published; no IG/MYC translocation detected

Viruses:

PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/-II -, MLV -

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