Nanomicro Technology는 고정밀 미세입자와 고성능 분리 재료를 전문으로 제조하는 회사로,
생명 과학, 제약, 및 화학 산업에서 고효율 분리와 분석 솔루션을 제공합니다.
제품 설명
NW Rose Series
제품 번호
Product Name | Pack Size | Cat. No. |
NW Rose Ni FF 5mL | 5mL | 60042-531800-2005 |
NW Rose Ni FF 25mL | 25mL | 60042-531800-2025 |
NW Rose Ni FF 100mL | 100mL | 60042-531800-2100 |
NW Rose Ni FF 200mL | 200mL | 60042-531800-2200 |
NW Rose Ni FF 200mL | 200mL | 60042-531800-2200 |
NW Rose Ni FF 500mL | 500mL | 60042-531800-2500 |
NW Rose Ni FF 1L | 1L | 60042-531800-1001 |
NW Rose Ni FF 5L | 5L | 60042-531800-1005 |
NW Rose Ni FF 10L | 10L | 60042-531800-1010 |
NW Rose Ni FF 20L | 20L | 60042-531800-1020 |
NW Rose TED FF 5mL | 5mL | 60042-371301-2005 |
NW Rose TED FF 25mL | 25mL | 60042-371301-2025 |
NW Rose TED FF 100mL | 100mL | 60042-371301-2100 |
NW Rose TED FF 200mL | 200mL | 60042-371301-2200 |
NW Rose TED FF 200mL | 200mL | 60042-371301-2200 |
NW Rose TED FF 500mL | 500mL | 60042-371301-2500 |
NW Rose TED FF 1L | 1L | 60042-371301-1001 |
NW Rose TED FF 5L | 5L | 60042-371301-1005 |
NW Rose TED FF 10L | 10L | 60042-371301-1010 |
NW Rose TED FF 20L | 20L | 60042-371301-1020 |
NW Rose IMAC FF 5mL | 5mL | 60042-092100-2005 |
NW Rose IMAC FF 25mL | 25mL | 60042-092100-2025 |
NW Rose IMAC FF 100mL | 100mL | 60042-092100-2100 |
NW Rose IMAC FF 200mL | 200mL | 60042-092100-2200 |
NW Rose IMAC FF 200mL | 200mL | 60042-092100-2200 |
NW Rose IMAC FF 500mL | 500mL | 60042-092100-2500 |
NW Rose IMAC FF 1L | 1L | 60042-092100-1001 |
NW Rose IMAC FF 5L | 5L | 60042-092100-1005 |
NW Rose IMAC FF 10L | 10L | 60042-092100-1010 |
NW Rose IMAC FF 20L | 20L | 60042-092100-1020 |
제품 특징
Immobilized metal affinity chromatography (IMAC) is an important tool for the purification of recombinant proteins with a cluster of histidine residues at the N- or C-terminus (also known as His-tagged proteins), or proteins naturally rich in histidine or cystine residues. Using IMAC, these proteins can be effectively captured from raw cell culture harvest followed by elution with an appropriate concentration of imidazole, and thus separated from impurity proteins and nucleic acids produced by the expression host cells. NanoMicro Technologies offers a wide variety of IMAC resins, pre-charged with Ni2+ or uncharged, for the preparation of highly pure recombinant proteins for scientific research, diagnostic assays and therapeutic applications.
Polymeric or cross-linked agarose particles with outstanding pressure-flow properties
Efficient mass transfer and high binding capacity; small elution volumes
Minimal non-specific binding; excellent His-tagged protein recovery
Low metal leaching level and excellent chemical compatibility
Uncharged resins available for the immobilization of divalent metals, such as Zn2+, Cu2+, Co2+, and Fe3+, for alternative protein binding selectivity
Product Name | NW Rose Ni FF | NW Rose TED FF | NW Rose IMAC FF |
| Chromatography Technique | Histidine-tagged protein purification | Histidine-tagged protein purification | Histidine-tagged protein purification |
| Matrix | Cross-linked agarose with fast flow rate | Cross-linked agarose with fast flow rate | Cross-linked agarose with fast flow rate |
| Particle Size | 45-165 μm | 45-165 μm | 45-165 μm |
| Ligand | NTA | TED | NTA |
| Dynamic Binding Capacity | ~40 mg·mL-1 | ~10 mg·mL-1 | ~40 mg·mL-1 |
| Metal Ion Capacity | 15 μmol·mL-1(Ni2+) | 45 μmol·mL-1(Ni2+) | ~15 μmol·mL-1(Ni2+) |
| Maximum Pressure | 0.3 MPa | 0.3 MPa | 0.3 MPa |
| Clean In Place | 0.5 M NaOH (Ni2+must be removed) | 0.5 M NaOH (Ni2+must be removed) | 0.5 M NaOH (Ni2+must be removed) |
| Maximum Flow Velocity | 150 cm/h（50/30 Column, bed height 10 cm） | 150 cm/h（50/30 Column, bed height 10 cm） | 150 cm/h（50/30 Column, bed height 10 cm） |
| Recommended Flow Velocity | 600 cm/h, 16/20 Column, bed height 10 cm | 600 cm/h, 16/20 Column, bed height 10 cm | 600 cm/h, 16/20 Column, bed height 10 cm |
| pH Stability | 3-12 (operational), 2-14 (CIP, Ni2+must be removed) | 3-12 (operational), 2-14 (CIP) | 3-12 (operational), 2-14 (CIP, Ni2+must be removed) |
| Chemical Stability | Stable in commonly used aqueous buffers, 0.1 M HCl, 0.1 M NaOH, 2% SDS, 30% isopropyl alcohol, 6 M guanidine hydrochloride, 8 M urea, 70% ethanol (if Ni2+ is removed) | Stable in commonly used aqueous buffers, 0.1 M HCl, 0.1 M NaOH, 2% SDS, 30% isopropyl alcohol, 6 M guanidine hydrochloride, 8 M urea, 70% ethanol (if Ni2+ is removed) | Stable in commonly used aqueous buffers, 0.1 M HCl, 0.1 M NaOH, 2% SDS, 30% isopropyl alcohol, 6 M guanidine hydrochloride, 8 M urea, 70% ethanol (if Ni2+ is removed) |
| Operational Temperature | 4-30℃ | 4-30℃ | 4-30℃ |
| Storage | 20% ethanol, 4-30 ℃ | 20% ethanol, 4-30 ℃ | 20% ethanol, 4-30 ℃ |
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