Product NameRaf1(Phospho-Ser259) Antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

ApplicationsWB IHC

Species ReactivityHu Ms Rt

SpecificityThe antibody detects endogenous level of Raf-1 only when phosphorylated at serine 259.

Immunogen TypePeptide-KLH

Immunogen DescPeptide sequence around phosphorylation site of serine 259 (S-T-S(p)-T-P) derived from Human Raf1.

Target NameRaf1

ConjugateUnconjugated

Other NamesC-RAF; C-Raf; CRAF

Accession NoSwiss-Prot: P04049
NCBI Protein: NP _002871.1

UniprotP04049

Gene ID5894;

Concentration1.0mg/ml

FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.

Predicted MW: 73kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100