(주)모아바이오

Biotechrabbit

[Biotechrabbit] RTS 100 E. coli HY Kit

Cat-No. BR1400106

Biotechrabbit은 바이오의약품 및 생명과학 연구를 위한 고급 실험 재료와 서비스를 제공하는 회사입니다.

제품 설명

RTS 100 E. coli HY Kit

제품 번호

BR1400106 - 6 reactions of 50 µl

BR1400101 - 24 reactions of 50 µl

BR1400102 - 96 reactions of 50 µl

제품 특징

Features

Compatible with linear templates for fast screening without cloning
Convenient reaction setup
Fast expression of proteins in parallel
Improved high yield biochemistry for up to 20 μg protein per reaction

Applications

Fast parallel microgram-scale protein expression

Description

The biotechrabbit RTS 100 E. coli HY Kit contains a modified high-yield E. coli lysate to drive coupled in vitro transcription and translation reactions. The enhanced kit can express up to 20 μg protein (50 μl reaction) in 4 hours using a batch mode. The RTS 100 E. coli HY kit is compatible with both linear templates and plasmids, making it the product of choice for protein-expression screening and optimization.

Each kit contains everything you need to perform protein-expression in the delivered microplate or, alternatively, in microtubes.

(former product codes: 24 rxn kit RiNA N-1101-24, 5PRIME 2401100 / 96 rxn kit RiNA N-1101-96, 5PRIME 2401110)

Other Information

Proteins expressed	Standard soluble proteins Membrane proteins
Reaction time / yield	1–4 h / 20 μg
Reaction mode	Batch
Template	RTS Linear Template Kit Plus RTS Linear Template Fab Kit RTS 100 E. coli LinTempGen Set, His RTS pIX3.0 Vector RTS pIVEX E. coli His-tag, 2nd Gen. V.

Quality Control

in vitro transcription/translation protein expression products are manufactured using quality chemicals and materials that meet our high standards. All product components are subjected to rigorous quality assurance testing process:

Component testing: each component is tested to ensure the composition and quality meet stated specifications.
Performance testing: each product is tested to ensure it meets the stated performance specification.

Protocols

The RTS 100 E. coli HY Kit is designed to turn genes into proteins within several hours, producing microgram yields to facilitate the assignment of biological functions to genes. Protein expression conditions can be optimized to enhance active protein production by adding chaperones, detergents, or other reagents to the reaction. The microplate format is easily adapted to high-throughput protein expression.

For detailed protocol, please, refer to the product manual (Related Documents).

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