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Biotechrabbit

[Biotechrabbit] RTS 500 ProteoMaster E. coli HY Kit

Cat-No. BR1400201

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제품 설명

RTS 500 ProteoMaster E. coli HY Kit

제품 번호

BR1400201

제품 특징

Features

Unique CECF technology to ensure high yields
Enable expression of toxic proteins that cannot be produced in vivo
Incorporation of labeled methionine into synthesized proteins

Applications

Optimized medium-scale, cell-free expression of up to 6 mg protein
Protein synthesis for NMR spectroscopy or X-ray crystallography

Description

The biotechrabbit RTS 500 ProteoMaster E. coli HY Kit is designed and optimized for the expression of up to 6 mg of protein from plasmid templates in highly efficient coupled transcription/translation reactions. This is achieved by utilizing a biochemically enhanced high-yield (HY) E. coli lysate and the RTS 500 CECF reaction device.

Due to preparative expression yields, the RTS 500 Proteo-Master E. coli HY Kit is suited for the production of protein for NMR spectroscopy or X-ray crystallography. The kit design allows to use amino-acid mixtures of your choice. Methionine (included in the kit separately from other amino acids) can be easily substituted by selenomethionine (not included). Other amino acids can be exchanged by preparing tailored amino-acid mixtures using the RTS Amino Acid Sampler.

The RTS 500 ProteoMaster E. coli HY Kit is optimized for use in a thermomixer. The continuous agitation of the device guarantees optimal support of the CECF process, which is required for efficient protein expression.

(former product codes: RiNA N-1203-50, 5PRIME 2401500)

Other Information

Proteins expressed	Standard soluble proteins Membrane proteins
Reaction time / yield	6–24 h / 6 mg
Reaction mode	CECF
Template	RTS pIX3.0 Vector RTS pIVEX E. coli His-tag, 2nd Gen. V.

Quality Control

in vitro transcription/translation protein expression products are manufactured using quality chemicals and materials that meet our high standards. All product components are subjected to rigorous quality assurance testing process:

Component testing: each component is tested to ensure the composition and quality meet stated specifications.
Performance testing: each product is tested to ensure it meets the stated performance specification.

Protocols

The RTS 500 ProteoMaster E. coli HY Kit is optimized for use in an Thermomixer in combination with the RTS 500 Adapter. The continuous agitation of the device guarantees optimal support of the CECF process, which is required for efficient protein expression.

For detailed protocol, please, refer to the product manual (Related Documents).

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