(주)모아바이오

Biotechrabbit

[Biotechrabbit] RTS 100 Wheat Germ Kit

Cat-No. BR1402501

Biotechrabbit은 바이오의약품 및 생명과학 연구를 위한 고급 실험 재료와 서비스를 제공하는 회사입니다.

제품 설명

RTS 100 Wheat Germ Kit

제품 번호

BR1402501

제품 특징

Features

Efficient expression of up to 5 μg protein per reaction
Compatible with non-optimized, pro- and eukaryotic T7 promoter-based vectors as well as linear templates
Excellent homogeneity of large proteins (264 kDa expressed)
No endotoxins, allows direct use in cell-based assays
Upscaling using high yield CECF wheat germ kits

Applications

Fast parallel synthesis for screening purposes
Synthesis of mammalian or other proteins for functional testing without the disturbing endogenous protein background that is typical for in vivo expression
Synthesis of proteins dependent on eukaryotic chaperones

Description

The biotechrabbit RTS 100 Wheat Germ Kit is a batch screening system allowing rapid protein expression within 2.5 hours. The kit allows use of a broad range of nonoptimized T7 promoter-based expression vectors from prokaryotic and eukaryotic origin in addition to established cell-free wheat germ vectors or linear templates. Using this approach, multiple constructs — including vectors that have not been optimized for wheat germ — can be screened rapidly for expression and functionality in parallel. The procedure saves precious time otherwise required for template generation. The new batch system yields up to 100 μg protein per ml reaction, which is sufficient for many downstream applications.

Other Information

Proteins expressed	Standard soluble proteins Complex and large proteins Membrane proteins
Reaction time / yield	3 h / 5 μg
Reaction mode	Batch
Template	RTS Wheat Germ LinTempGen Set RTS pIVEX Wheat Germ His₆-tag Vector

Quality Control

in vitro transcription/translation protein expression products are manufactured using quality chemicals and materials that meet our high standards. All product components are subjected to rigorous quality assurance testing process:

Component testing: each component is tested to ensure the composition and quality meet stated specifications.
Performance testing: each product is tested to ensure it meets the stated performance specification.

Protocols

The RTS 100 Wheat Germ Kit enables high success rates for expression and solubility, particularly for eukaryotic target proteins. Synthesize proteins in batch format within 2.5 hours using a broad range of non-optimized T7 promoter-based expression vectors of pro- and eukaryotic origin. Convenience is greatly improved, due to the ability to use PCR-generated linear templates in addition to plasmid templates. Use this kit to synthesize proteins in a wide molecular weight range (up to 264 kDa tested), as well as to synthesize truncated protein variants (e.g., for epitope or functional domain mapping).

For detailed protocol, please, refer to the product manual (Related Documents).

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