(주)모아바이오

Biotechrabbit

[Biotechrabbit] RTS 100 Wheat Germ CECF Kit

Cat-No. BR1401001

Biotechrabbit은 바이오의약품 및 생명과학 연구를 위한 고급 실험 재료와 서비스를 제공하는 회사입니다.

제품 설명

RTS 100 Wheat Germ CECF Kit

제품 번호

BR1401001

제품 특징

Features

Successful expression of eukaryotic proteins , generally without sequence optimization
Synthesis of up to 50 µg functional eukaryotic protein
Maximized production of active heat-sensitive proteins, due to an optimum reaction temperature of 24°C

Applications

Up to milligram-scale protein expression of functional eukaryotic proteins
Expression of multiple proteins in parallel

Description

The biotechrabbit RTS 100 Wheat Germ CECF Kit enables high success rates for expression and solubility, particularly for eukaryotic target proteins. The use of PCR-generated linear templates greatly improves convenience. The kits allow synthesis of proteins in a wide molecular weight range (up to 220 kDa, as tested), as well as synthesis of truncated protein variants (e.g., for epitope or functional domain mapping). While the RTS 100 Wheat Germ CECF Kit is designed for high-throughput protein expression, the RTS 500 version ensures higher yields.

The system can be used with linear templates as well as mRNA, vectors and plasmid DNA. Depending on the protein, expression yields of more than 1 mg protein per reaction can be obtained within 24 hours in a 1 ml reaction.

(former product codes: RiNA N-2201-24, 5PRIME 2402100)

Other Information

Proteins expressed	Standard soluble proteins Complex and large proteins Membrane proteins
Reaction time / yield	6–24 h / 50 μg (50 μl)
Reaction mode	CECF
Template	RTS Wheat Germ LinTempGen Set RTS pIVEX Wheat Germn His6-tag Vect.

Quality Control

in vitro transcription/translation protein expression products are manufactured using quality chemicals and materials that meet our high standards. All product components are subjected to rigorous quality assurance testing process:

Component testing: each component is tested to ensure the composition and quality meet stated specifications.
Performance testing: each product is tested to ensure it meets the stated performance specification.

Protocols

The RTS 100 Wheat Germ CECF Kit enables high success rates for expression and solubility, particularly for eukaryotic target proteins. Convenience is greatly improved, due to the ability to use PCR-generated linear templates; plasmids and mRNA can also be used as template. Use this kit to synthesize proteins in a wide molecular weight range, as well as to synthesize truncated protein variants (e.g., for epitope or functional domain mapping). The microplate format is easily adapted to high-throughput protein expression and makes this an ideal kit for screening applications.

For detailed protocol, please, refer to the product manual (Related Documents).

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