(주)모아바이오

Biotechrabbit

[Biotechrabbit] RTS pIVEX E. coli His-tag, 2nd Gen. Vector Set

Cat-No. BR1400701

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제품 설명

RTS pIVEX E. coli His-tag, 2nd Gen. Vector Set

제품 번호

BR1400701

제품 특징

Features

Stable circular templates to ensure high yields in preparative-scale expressions
For construction of C- and N-terminal His6-tagged proteins
Optimal spacing of all regulatory elements
For use with RTS E. coli and Insect kits

Applications

Preparative-scale expression of His6-tagged proteins

Description

The RTS pIVEX E. coli vectors are designed for high-yield cell-free expression of His6-tagged proteins using a broad range of RTS expression kits.

The two vectors contain all regulatory elements necessary for in vitro expression based on a combination of T7 RNA polymerase and RTS expression lysates. They enable the attachment of a His6-tag in different orientations at the C- and N-terminus of the protein. Cloning into RTS pIVEX His6-tag vectors allows optimal protein expression with high yields.

(former product codes: RiNA N-3110-02, 5PRIME 2401010)

Other Information

Protein tag	His tag
Expression kits	RTS 100 E. coli HY Kit RTS 500 ProteoMaster E. coli HY Kit RTS 9000 E. coli HY Kit RTS 100 E. coli Fab Kit RTS 100 E. coli Disulfide Kit RTS 500 E. coli Disulfide Kit RTS 100 Insect Membrane Kit RTS 100 Site-Specific Label Kit

Quality Control

in vitro transcription/translation protein expression products are manufactured using quality chemicals and materials that meet our high standards. All product components are subjected to rigorous quality assurance testing process:

Component testing: each component is tested to ensure the composition and quality meet stated specifications.
Performance testing: each product is tested to ensure it meets the stated performance specification.

Protocols

Each pIVEX vector included in this product encodes a hexa-histidine fusion tag to allow easy detection and purification of the expressed protein. pIVEX 2.3d is used for fusing genes to a C-terminal His6-tag, pIVEX 2.4d is used for fusing genes to an N-terminal His6-tag. For expression without a tag, pIVEX 2.3d can be used in combination with the addition of a stop codon at the end of the gene.

For detailed protocol, please, refer to the product manual (Related Documents).

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