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[HOPAX] BIS-TRIS Buffer

Cat-No. 6976-37-0

Hopax는 생물학용 버퍼, 양쪽성(detergents), 전기도금 및 리튬이온 배터리 첨가제 등 정밀화학 제품을 전문적으로 생산하는 글로벌 기업입니다.

제품 설명

BIS-TRIS Buffer

제품 번호

6976-37-0

제품 특징

Applications：

Cell culture media | Chromatography | Electrophoresis | Protein purification

Description

Assay (titr.): ≥ 99% | CAS No: 6976-37-0 | Useful pH range: 5.8 – 7.2

Hopax is a manufacturer and supplier of BIS-TRIS (CAS 6976-37-0), a zwitterionic biological buffer often used as a buffering agent in biological and biochemical research.

Uses of BIS-TRIS Buffer:

BIS-TRIS is a popular gel, sample and running buffer for various types of electrophoresis. For instance, it has been used with ACES Buffer as a running buffer for gel electrophoresis (BIS-TRIS/ACES).
Its use has also been reported in anion exchange chromatography, NMR spectroscopy and x-ray crystallography.
This Good's Buffer is considered an excellent substitute for the highly toxic buffer cacodylate.

Concerns:

It is not suitable for use with the Bicinchoninic Acid (BCA) Assay.
It interacts with human liver fatty acid binding protein (FAB) and affects protein dynamics.

Metal binding: (Compare with other buffers)

Strong interaction with:	Cu and Pb
Weak interaction with:	Mg, Ca, Mn, Co, Ni, Zn and Cd

Solubility in water: (Compare with other buffers)

20g/100ml

Storage:

Store at room temperature

Basic Information

Specifications:

Assay (titr.)	≥ 99%
Useful pH range	5.8 – 7.2 Compare with other buffers
pKa (25°C)	6.3 – 6.7
Appearance	White powder
Molecular formula	C₈H₁₉NO₅
Molecular weight	209.2 g/mol

pH (1% in H2O)

8.8 – 9.8

IR	In conformance with known standard

UV (0.1M in H2O)A280nm

≦ 0.04

UV (0.1M in H2O)A340nm

≦ 0.02

≦ 3 ppm

Synonyms / Similar names:

2,2-Bis(hydroxymethyl)-2,2′,2″-nitrilotriethanol
2-Bis(2-hydroxyethyl)amino-2-(hydroxymethyl)-1,3-propanediol
Bis(2-hydroxyethyl)amino-tris(hydroxymethyl)methane

What we offer:

Certificate of Analysis (CoA)
Small and bulk quantities available in stock
Analytical tests (DNase, RNase, protease activity, heavy metals and others upon request)

Useful pH range chart

Use our interactive chart below to compare the useful pH range of this product with other buffers’. Click in the product name to access more information about it.