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제품 설명
Hieff UNICON™ ColorGPS qPCR Master Mix (No Rox)
제품 번호
11188ES
제품 설명
Hieff UNICON™ ColorGPS qPCR Master Mix (No Rox) is a pre-solution for 2×real-time quantitative PCR amplification, featuring high fluorescence intensity, high sensitivity and specificity, and high amplification yield. The core component, Hieff UNICON™ Taq DNA Polymerase, is hot-started by antibody method, which can effectively inhibit non-specific amplification caused by primer annealing during sample preparation. At the same time, the formula adds factors to enhance the amplification efficiency of the PCR reaction and facilitators to equalize the amplification of genes with different GC contents (30~70%), so that the quantitative PCR can obtain a good linear relationship in a wide range of quantitative regions. The product utilizes the mixed color change reaction of different dyes to monitor the pipetting operation, which effectively reduces the occurrence of pipetting errors.
Excellent Amplification: Good linearity across a wide linear range
High Precision: Can accurately distinguish templates with a 2-fold concentration difference
Good Repeatability: Small inter-well variability in replicate wells, resulting in better repeatability
Stable and Reliable: The reaction system can be stored at room temperature for 24 hours, and the product remains unaffected after 20 freeze-thaw cycles
Concentration | 2 ×(qPCR Mix) |
Detection method | Dye method |
PCR method | qPCR |
Polymerase | Taq DNA Polymerase |
Type of sample | DNA |
Application equipment | Bio-Rad CFX96, CFX384, iCycler iQ, iQ5, MyiQ; MiniOpticon, Opticon, Opticon 2, Chromo4; Cepheid SmartCycler; Eppendorf Mastercycler ep realplex, realplex 2 s; Illumina Eco qPCR; Qiagen/Corbett Rotor-Gene Q, Rotor-Gene 3000, Rotor-Gene 6000;Roche Applied Science LightCycler 480; Thermo Scientific PikoReal Cycler and other fluorescence quantitative PCR instruments that do not require the addition of ROX Reference Dye |
Product Type | Premix for real-time fluorescence quantitative PCR |
Apply to (application) | Gene Expression |
Components No. | Name | 11188ES03 | 11188ES08 | 11188ES60 |
11188-A | Hieff UNICON™ ColorGPS qPCR Master Mix (No Rox) | 1 mL | 5×1 mL | 100×1 mL |
11188-B | 10×Dilution Buffer | 1 mL | 1 mL | 20×1 mL |
This product should be stored at -25~-15℃ for 1 years.
Gene Expression Difference Analysis
Absolute Quantitative Analysis
Figure1. Reliable results can be obtained within the dynamic range.
Using 2 µL of plasmid templates with a gradient of 10^1-10^7, the related genes were amplified. Hieff UNICON™ ColorGPS master mix can effectively detect a template amount range of 7 orders of magnitude, achieving good linearity within a broad linear range.
Figure2. Can accurately distinguish template concentration differences of 2-fold.
Using 2 µL of plasmid diluted in a 2-fold gradient as the template, the related genes were amplified. Hieff UNICON™ ColorGPS master mix can accurately resolve template concentration differences.
Figure3. Small inter-well variability is observed, showing better repeatability.
Using dozens of copies of plasmid as the template, amplification was performed with different fluorescent quantitative reagents according to the procedures specified in their respective instruction manuals. The SD values indicate that, compared to other reverse transcription reagents, the new product has smaller differences between replicate wells and better repeatability.
Figure4. More flexible, suitable for standard or rapid procedures.
In the standard procedure, the denaturation time and extension time are set to 10 seconds and 30 seconds, respectively, while in the rapid procedure, the denaturation time and extension time are set to 3 seconds and 10 seconds, respectively, to detect the Ct values corresponding to 10 target genes. The results show that the Ct values obtained with the new product in both standard and rapid procedures have a strong correlation, and the new product can achieve good results using the rapid procedure.
Figure5. After the reaction system is prepared, it can remain stable at room temperature for 24 hours.
Using different genes from mouse, human 293 cells, and Arabidopsis thaliana as targets, after preparing the reaction system, it was placed in the dark at different temperatures for 24 hours before detection. Compared to immediate processing, the detection difference for different genes does not exceed 0.5 Ct, indicating that stable results can also be obtained with long-duration operations.
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