To generate a recombinant monoclonal antibody against L1CAM, CUSABIO initiated the process by immunizing a rabbit with a synthesized peptide derived from human L1CAM. B cells were subsequently isolated from the immunized rabbit, and RNA was extracted from these cells. The extracted RNA was reverse-transcribed into cDNA, which was then used as a template to extend L1CAM antibody genes using degenerate primers. These synthesized L1CAM antibody genes were incorporated into a plasmid vector and transfected into host cells for expression. The resulting L1CAM recombinant monoclonal antibody was i...Read more