To generate a recombinant monoclonal antibody specific to ARG1, the initial step involved immunizing a rabbit with a synthesized peptide derived from human ARG1 protein. B cells were subsequently isolated from the immunized rabbit, and RNA was extracted from these cells. The extracted RNA was reverse-transcribed into cDNA, which was utilized as a template to extend ARG1 antibody genes using degenerate primers. These engineered ARG1 antibody genes were incorporated into a plasmid vector and transfected into host cells for expression. The resulting ARG1 recombinant monoclonal antibody was iso...Read more