To create a recombinant monoclonal antibody against SOD2 acetylated at K68, CUSABIO's approach began with the immunization of a rabbit using a synthesized peptide derived from human SOD2 protein. Subsequent steps involved isolating B cells from the immunized rabbit and extracting RNA from these cells. The extracted RNA was reverse-transcribed into cDNA, which was utilized as a template for extending SOD2 antibody genes using degenerate primers. These extended SOD2 antibody genes were integrated into a plasmid vector and introduced into host cells for expression. The acetyl-SOD2 (K68) recomb...Read more