YEASEN은 생명과학 연구 및 진단을 위한 고품질의 연구용 시약과 기기를 제공하는 글로벌 바이오 기업입니다.
제품 설명
Recombinant Human FGF basic/FGF2/bFGF, GMP Protein
제품 번호
91341ES10
제품 설명
Basic fibroblast growth factor (bFGF) is a member of the fibroblast growth factor (FGF) family, which is a growth factor and signaling protein encoded by the FGF2 gene. FGF family members have a wide range of activities that promote mitosis and cell survival, and are involved in various biological processes including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth, and invasion. In normal tissues, bFGF exists in the extracellular matrix beneath the basement membrane and vascular endothelium. bFGF can stimulate and regulate the differentiation and proliferation of various cells originating from the mesoderm and neuroectoderm, including vascular endothelial cells, epithelial cells, myoblasts, osteoblasts, and glial cells. FGF-2 plays an important role in embryonic development and tissue healing. bFGF is the fundamental component that maintains pluripotency in all embryonic and induced pluripotent stem cell culture media.
The product is expressed in E. coli and provided in liquid form, with high activity, high purity and low endotoxin.
Synonyms | bFGF, FGF basic, FGF2, FGF-2, fibroblast growth factor 2 (basic), HBGF-2, Prostatropin. |
Source | Human bFGF Protein is expressed from E.coli without tag. |
Sequence | Ala 144 - Ser 288. |
Molecular Weight | Approximately 16.29 kDa. |
Accession | P09038. |
Biological Activity | Human bFGF GMP Protein stimulates proliferation of murine balb/c 3T3 cells. The specific activity of Human bFGF GMP Protein is >8.0 x 10^5 IU/mg, which is calibrated against Basic Fibroblast Growth Factor WHO International Standard (NIBSC code: 90/712). |
Purity | ＞95% as determined by SDS-PAGE and HPLC. |
Endotoxin | ＜0.01 EU/μg protein as determined by LAL method. |
Sterility | Negative. |
Mycoplasma | Negative. |
Vitro Virus | Negative. |
Host Cell Protein | Less than 0.5 ng/μg when tested by ELISA. |
Host Cell DNA | Less than 20 ng/mg when tested by qPCR. |
Formulation | Liquid from 0.22 μm filtered solution in PBS. |
Physical Appearance | Liquid. |
The product should be stored at -85~-65℃ for 2 years.
1.Please operate with lab coats and disposable gloves, for your safety.
2.This product is for research use only.
SDS-PAGE
Figure 1. The purity is greater than 95% as determined by SDS-PAGE under reduced condition.
SEC-HPLC
Figure 2. The purity is greater than 95% as determined by SEC-HPLC.
Cell Based Assay
Figure 3. Human bFGF GMP Protein stimulates proliferation of murine balb/c 3T3 cells. The specific activity of Human bFGF GMP Protein is >8.0 x 10^5 IU/mg, which is calibrated against Basic Fibroblast Growth Factor WHO International Standard (NIBSC code: 90/712).
YEASEN의 모든 제품을 만나 보세요!
YEASEN - Official Distributor in South Korea "Morebio" 한국 공식 대리점 "모아바이오"
MSDS
온라인 견적
