YEASEN은 생명과학 연구 및 진단을 위한 고품질의 연구용 시약과 기기를 제공하는 글로벌 바이오 기업입니다.
제품 설명
Hieff Trans™ Booster DNA/RNA Transfection Reagent
제품 번호
20418ES
제품 설명
Kex2 protease, also known as a double-alkaline endopeptidase or YSCF protease, is a calcium-dependent serine protease that is responsible for processing the precursors of killer toxin and α-factor within yeast cells. It specifically recognizes and cleaves the peptide bonds at the carboxyl end of dibasic amino acids such as Arg-Arg and Lys-Arg. Unlike trypsin, Kex2 does not recognize and cleave the peptide bonds at the carboxyl end of single basic amino acids, such as arginine or lysine. The activity of Kex2 protease is not inhibited by conventional serine protease inhibitors such as PMSF, TPCK.
YeasenUCF.METM Recombinant Kex2 Protease is expressed in Pichia pastoris, produced under GMP regulations, free of any animal-derived components, and without the risk of viral contamination from animal sources. It has the same enzymatic specificity as the natural Saccharomyces cerevisiae Kex2 enzyme. The optimal pH for activity is pH 9.0, and the stable storage pH is between 5.0 and 6.0.
Strong specificity—Specifically recognizes and cleaves the peptide bonds at the carboxyl end of dibasic amino acids such as Arg-Arg and Lys-Arg.
High purity—Purity≥95%.
Animal free—Recombinantly produced, free from exogenous viral contamination, and no animal-derived materials are used in the production process.
Processive enzymatic cleavage in peptide drug production.
Enzymatic digestion of proteins and peptide mapping, sequencing, etc.
The products are shipped with ice pack and can be stored at -20℃ to -80℃ for 1 year.
Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.
Source | Yeast recombinant expression |
Molecular Weight | Theoretical value 67.0±6.7 kDa |
Appearance | White or off-white powder |
Enzyme Concentration | ≥10.0 units/mg pro |
Unit Definition | At 25℃, in a 3 mL reaction system containing 50 mM Tris-HCl and 2 mM CaCl2 at pH 8.0, the amount of enzyme that catalyzes the release of 1 μmoL of 4-nitroaniline from the substrate Boc-QRR-pNA per minute is defined as one unit of enzyme activity. |
Quality Assurance | SDS-PAGE gel detection shows a clear single band of the target protein; no other proteases present, no non-specific cutting. |
Components No. | Name | 20418ES60 | 20418ES80 | 20418ES90 | 20418ES91 | 20418ES92 |
20418 | UCF.METM Recombinant Kex2 Protease（Powder） | 100 μg | 1 mg | 10 mg | 100 mg | 1 g |
Lyophilized powder can be stored at 2~8℃ for one year, protected from light and moisture.
Dissolution and Storage Buffer (20 mM NaAc-HAc, pH 5.2, 2 mM Ca2+), after dissolution, store at below -20℃, it is recommended to aliquot for storage to avoid repeated freezing and thawing.
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