(주)모아바이오

YEASEN

[YEASEN] TOP10 Chemically Competent Cell

Cat-No. 11801ES

11801ES80 / size: 10×100 μL
11801ES92 / size: 100×100 μL

YEASEN은 생명과학 연구 및 진단을 위한 고품질의 연구용 시약과 기기를 제공하는 글로벌 바이오 기업입니다.

제품 설명

TOP10 Chemically Competent Cell

제품 번호

11801ES80 / size: 10×100 μL

11801ES92 / size: 100×100 μL

제품 설명

Description

TOP10 Chemically Competent Cell from YEASEN is the competent cells obtained by the special process of Escherichia coli strain TOP10 with Streptomycin (StrR) resistance. It can be used for the chemical transformation of DNA with a conversion efficiency of up to 10⁸ cfu/μg plasmid DNA. It is widely used for efficient DNA cloning and plasmid amplification, and can ensure stable inheritance of high-copy plasmids. At the same time, it can be used to construct clones and blue-white colony screening.

TOP10 competent cell genotypes: F-mcrA △(mrr-hsdRMS-mcrBC) φ80lacZ△M15 △lacX74 recA1 ara△139 △(ara-leu) 7697 galU galK rpsL (StrR ) endA1 nupG.

Features

High transformation efficiency: >1×10⁸ cfu/μg pUC19 DNA
recA1 was used to reduce nonspecific recombination in cloned DNA
lacZΔM15 was used for blue/white colony screening of recombinant clones

Applications

Efficient cloning
Plasmid proliferation

Specifications

Species	Escherichia coil
Cell type	Chemical competent cell
Whether it contains an F’ episome	No
Efficiency	>1×10^8
Blue-white colony screening	Yes
Bacterial or yeast strains	TOP10
Amplification of the ccdB-containing vector	It is not suitable for the amplification of ccdB-containing vectors