(주)모아바이오

YEASEN

[YEASEN] DH5α Chemically Competent Cell

Cat-No. 11802ES

11802ES80 / size: 10x100μl
11802ES92 / size: 100x100μl

YEASEN은 생명과학 연구 및 진단을 위한 고품질의 연구용 시약과 기기를 제공하는 글로벌 바이오 기업입니다.

제품 설명

DH5α Chemically Competent Cell

제품 번호

11802ES80 / size: 10x100μl

11802ES92 / size: 100x100μl

제품 설명

Description

DH5α Chemically Competent Cell from YEASEN is a competent cell obtained by a special process of DH5α strain. It can be used for the chemical transformation of DNA with a conversion efficiency of up to 10⁸ cfu/μg plasmid DNA. It is widely used for efficient DNA cloning and plasmid amplification and can ensure the stable inheritance of high-copy plasmids. At the same time, it can be used to construct clones and blue-white spot screening. Deletion of recA1 and endA1 genes in DH5α cells ensured the stability of cloned DNA.

DH5α competent cell genotypes: F- φ80 lac ZΔM15 Δ(lacZYA-arg F) U169 endA1 recA1 hsdR17(rk-,mk+) supE44λ- thi -1 gyrA96 relA1phoA

Features

High transformation efficiency: >1×108 cfu/μg pUC19 DNA
Genetic markers that allow for blue/white colony screening

Applications

Efficient DNA cloning derived from PCR, cDNA-generation reactions
recA1 marker facilitates working with difficult-to-transform DNA

Specifications

Species	Escherichia coil
Cell type	Chemical competent cell
Whether it contains an F’ episome	No
Efficiency	>1×10^8
Blue/white colony screening	Yes
Bacterial or yeast strains	DH5α
Amplification of the ccdB-containing vector	It is not suitable for the amplification of ccdB-containing vectors