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제품 설명

Serum Free* CHO Cell Basal media_CHO MaxA

제품 번호

Serum Free*

제품 특징

Applicable cell lines

This medium is suitable for use as a basal medium for CHO-K1, CHO-S and CHO-DG44 cells in batch and fed-batch culture processes.

Medium characteristics  

1. This product is a chemically defined medium (CDM) which is Animal-Derived Component-Free (ADCF). 

2. This product contains amino acids, vitamins, glucose, inorganic salts, and trace elements and is free of growth factors, peptides, hydrolysates, and phenol red. 

3. The dry powder medium contains P188, without HT and L-glutamine.

Storage

1. Dry powder medium: Store at 2 to 8 ℃, away from light. 

2. Liquid medium: Store at 2 to 8 ℃, away from light.

Culture conditions

1. The cultivation temperature is 36.5℃. 

2. In batch and batch fed-batch culture processes, the recommended initial cell seeding density is (0.5 to 1) × 106 cells/mL. 

3. Under non-pH automatic control conditions, the CO2 concentration shall be controlled at 5%. 

4. Process conditions such as pH, DO and temperature can be set according to process development results or platform process parameters.

Cell cryopreservation 

When the conditioned medium made of this medium is used for cell cryopreservation, 5% to 10% DMSO shall be added, and the cell cryopreservation density shall be 1.0 to 2.0×107 cells/mL. 

Preparation method

1. Measure (weigh) the ultrapure water (18 to 25 ℃) of 80% of the final volume (weight) of the prepared liquid medium in the designated container, slowly add 22.22 g/L of CHO MaxA dry powder, and stir vigorously for 20 to 30 min until the dry powder is completely dissolved into a suspension. 

2. Slowly add 5 moL/L of sodium hydroxide solution per the amount of 6.5 mL/L and stir for 5 to10 min, Until the solution is completely clear. 

3. Add sodium bicarbonate powder per the amount of 1.8 g/L and stir for 5 to 10 min until the sodium bicarbonate is completely dissolved. 

4. Use 12 moL/L concentrated hydrochloric acids to adjust the pH of the liquid medium to 6.7 to 6.8, and stir for 3 to 5 min. 

5. Fix the volume to 1 L with ultrapure water (quantify to 1 kg) and continue to stir for 3 to 5 min. 

6. Determine the final pH (6.7 to 7.4) and osmotic pressure (270 to 330 mOsmol/kg) of the culture medium. 

7. 0.22μm membrane filtration.

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