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yocon

[yocon] Hybridoma Cells Serum Free Medium

Cat-No. NC0301



yocon Biology는 혈청 없는 세포 배양 배지(serum-free media) 및 분자 진단(molecular diagnostics) 제품을 연구·개발·공급하는 선도기업입니다.






제품 설명


Hybridoma Cells Serum Free Medium




제품 번호


NC0301




제품 특징


Hybridoma Cells Serum Free Medium

This product is suitable for hybridoma cell culture to get specific monoclonal antibodies.

Product use

It is suitable for hybridoma cell culture to get specific monoclonal antibodies, with high antibody yield and repeatability. Without animal-derived components. It is suitable for expansion culture in static condition, shake flask, culture bag and bioreactor according to a variety of production scales to meet the needs of different customers.


Catalog No.Product Name
Packaging SpecificationsStorage ConditionsShelf Life
NC0301Hybridoma cell serum-free culture medium1000mL/Bottle2-8℃, store away from light12months
NC0301.SAnti-augmentation agent100mL/Bottle2-8℃, store away from light12months


Anti-augmentative usage steps:

When the serum-free cultured hybridoma cells grow to the logarithmic growth phase, the day before the peak, add an anti-increasing agent according to the volume of the medium. If the medium is less than 100mL, add an anti-increasing agent to 100uL, and continue to culture after adding, when the cell viability drops to 30% to 40%, the supernatant can be harvested for antibody detection.


Precautions:

When using the anti-enhancing agent for the first time, please strictly follow the concentration recommended by our company. Generally, the antibody can be increased by 20% to 50%. The stimulating effect of different cell antibodies is different, and the optimal concentration needs to be further explored for different cell lines.


Use and Description

This medium can be used to culture hybridoma cells to obtain specific monoclonal antibodies.

Hybridoma cells are a fusion of lymphocytes (B cells) from immunized mice and mouse myeloma cells. The cells not only have the characteristics of cells that can produce specific antibodies but also have the unlimited proliferation ability of myeloma cells. It is a commonly used cell line for the production of monoclonal antibodies. The hybridoma serum-free culture medium has a clear chemical composition, does not add serum or serum substitutes (platelet lysate), and does not contain any animal-derived components. The difference between product batches is small, and all raw materials comply with GMP standards. More suitable for clinical research use.


Product advantages

Supporting hybridoma multi-cell line culture

This product can be used for the culture of mouse, rat and hamster hybridoma cells, which in most cases can be directly transferred without domestication

Stable antibody expression

The antibody concentration is greater than 300 mg/L under static culture condition and greater than 500 mg/L under dynamic culture condition, and the cell doubling time is on average one day earlier than that of its competitors

Suitable for mass production

This product is suitable for the culture in shake flasks, culture bags and bioreactors to meet different scales of production and R&D




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Cell Culture Media

Molecular Diagnostic



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