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제품 설명

NUGC-4

제품 번호

ABC-TC0862

제품 특징

Description

NUGC-4 is a human gastric cancer cell line derived from a 35-year-old Japanese female diagnosed with poorly differentiated adenocarcinoma containing signet ring cell carcinoma components in the metastatic paragastric lymph nodes. The cells are characterized by a spherical, epithelial-like morphology and exhibit adhesion properties, although occasional free-floating cells can be observed. They also display anchorage-independent growth, making them suitable for tumorigenicity studies. Typically cultured in PRMI1640 with 10% FBS, they show an average doubling time of 30 hours. The cells have a modal chromosome number of 52–54 in vitro and 53 in vivo. NUGC-4 cells do not express IL-1α mRNA, offering a unique gene expression profile relevant to inflammation and tumor microenvironment studies.

Citation

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Application

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    • • As a cancer cell line, NUGC-4 is widely used to identify key factors in gastric cancer formation and targets for anti-cancer drugs.Epidermal growth factor (EGF) strongly activates the tyrosine phosphorylation in NUGC-4. Phosphatidylinositol 3-kinase (PI 3-kinase), mainly activated by tyrosine kinases, was precipitated with anti-phosphotyrosine antibodies.Exogenous hepatocyte growth factor can stimulate the proliferation of NUGC-4, and MET tyrosine kinase inhibitors inhibited this proliferation of NUGC-4.Investigation of NUGC-4 and its interactions with growth factors may guide gastric cancer signaling pathways and therapeutic target identification..

Frequently Asked Questions

• What are NUGC-4 cells and their primary use in research?

  NUGC-4 cells are a human gastric cancer cell line derived from a poorly differentiated adenocarcinoma. They are widely used in cancer research, particularly for studies focused on gastric carcinoma, drug screening, and understanding cancer cell biology.

• What are common issues encountered with NUGC-4 cell culture and how can I troubleshoot them?

  Slow Growth: Ensure regular medium change, check for contamination, and avoid over-confluency.

  Poor Attachment: Use properly coated culture flasks and high-quality serum.

  Cell Death: Maintain optimal culture conditions, verify reagents are non-toxic, and handle cells gently.

• What is the morphology Characteristics of NUGC-4 cells?

  Cell Shape: NUGC-4 cells are epithelial-like in morphology. They typically appear as polygonal or slightly elongated cells when observed under a microscope.

  Adherence: They adhere well to the surface of culture flasks, forming a cobblestone-like pattern characteristic of epithelial cells.

  Cytoplasm and Nucleus: The cells have a well-defined cytoplasm and prominent nuclei, which are indicative of their active proliferative state.

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