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제품 설명
Bovine Non-esterified fatty acid (NEFA) Elisa Kit
제품 번호
EK760557
제품 특징
SKU: | EK760557 |
Category: | |
Species: | Bovine |
Assay Principle: | Quantitative |
Assay Type: | Sandwich |
Target Name: | Non-esterified fatty acid |
Sample Type: | serum , plasma , urine , tissue homogenates , cell culture supernates |
Sensitivity: | 0.8 nmol/mL |
Standard: | 360 nmol/mL |
Detection Range: | 4 nmol/ml - 300 nmol/ml |
Assay Time: | 1 - 5 h |
Sample Volume: | 50 - 100 ul |
Detection Wavelength: | 450 nm |
Abbreviation: | NEFA |
시약 준비 과정
Tube | 6 | 5 | 4 | 3 | 2 | 1 | 0 |
nmol/mL | 360 | 240 | 160 | 80 | 40 | 20 | 0 |
결과 값 계산
Take the standard concentration as the horizontal, the OD value for the vertical, draw the standard curve on graph paper, find out the corresponding concentration according to the sample OD value by the sample curve, multiplied by the dilution multiple, or calculate the straight line regression equation of the standard curve with the standard concentration and the OD value, with the sample OD value in the equation, calculate the sample concentration, multiplied by the dilution factor, the result is the sample actual concentration.
Test effectiveness: the average value of positive control ≥1.00; the average value of negative control ≤0.10. The critical value (CUT OFF) calculation: critical value = the average value of negative control + 0.15
Negative result : if the OD value
Positive result: if the OD value ≥ CUT OFF, the sample is positive.
Graphical Representation as follows
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