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YEASEN

[YEASEN] InStab ™ Protease Inhibitor Cocktail, EDTA-free, 100×DMSO Stock Solution

Cat-No. 20124ES


YEASEN은 생명과학 연구 및 진단을 위한 고품질의 연구용 시약과 기기를 제공하는 글로벌 바이오 기업입니다.






제품 설명


InStab ™ Protease Inhibitor Cocktail, EDTA-free, 100×DMSO Stock Solution




제품 번호

 

20124ES




제품 설명


Description

After cell lysis, the protein crude extract contains a large amount of endogenous proteases that can degrade proteins in the cell extract. To prevent the degradation of the target protein by proteases during protein purification, protease inhibitors are required to inhibit their activity. This product is a mixture of various common protease inhibitors, including AEBSF, Aprotinin, Bestatin, E-64, Leupeptin, Pepstatin A, and other components, which broadly inhibit a variety of proteases from plants, animals, bacteria, yeast, and other tissues or cells, such as serine proteases, cysteine proteases, aminopeptidases, and acidic proteases. Additionally, this product does not contain EDTA (a metalloproteinase inhibitor), making it particularly suitable for proteins whose active centers depend on metal ions. It is compatible with subsequent immobilized metal-chelate affinity chromatography (IMAC), 2-D gel electrophoresis, and native gel electrophoresis for protein analysis experiments.

This product is provided as a 100× stock solution dissolved in DMSO and can be used for WB, Co-IP, Pull-down assay, IF, IHC, kinase research, and other analyses.


Shipping and Storage

The product is shipped with ice pack and can be stored at -15~-25℃ for 1 year.


Components

Components No.

Name

20124ES03

20124ES10

20124ES60

20124

InStab ™ Protease Inhibitor Cocktail,EDTA-free,100×DMSO Stock Solution

1 mL

10×1 mL

100×1 mL


Instructions

Before use, allow the product to warm to room temperature. Add to the sample solution at a ratio of 1:100 (v/v) before sample analysis, and mix well.


Notes

1. This product has broad-spectrum protease inhibitory activity but does not possess phosphatase inhibitory activity. For research on phosphorylated proteins, our phosphatase inhibitor mixture (100×) (Cat#20109) can be used.

2. Please wear the necessary PPE, such lab coat and gloves, to ensure your health and safety

3. For research use only!




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