Recombinant Vesicular Stomatitis Virus (rVSV) pseudotyped with Bundibugyo ebolavirus glycoprotein (rVSV-BDBV-GP) system in which the G protein of VSV has been deleted, replaced with firefly luciferase and used to produce VSV-pseudotyped virus containing the envelope glycoprotein of Bundibugyo ebolavirus virus. Since the infectivity of pseudotyped virus is restricted to a single round of replication, analyses of viral entry can be performed using just biosafety level 2 (BSL-2) containment.

• Formulation & Storage: Supplied in EMEM™ (Gibco) supplemented with 1% Fetal Bovine Serum, L-glutamine and Penicillin/Streptomycin. Store at -80°C.
• Sequence: PP_006XHL9.2
• Notes & Usage Guidelines:
• Recommended Dilution: 1:40
• Luciferase Units for Assays: 30,000
• Reporter: Luciferase