DSMZ no.:

Species:

human (Homo sapiens)

Cell type:

neuroblastoma

Origin:

established in 1983 from an 1 year-old girl with neuroblastoma; described to carry an ALK and a TP53 mutation and a ca. 100-fold genomic amplification of N-MYC in der(17)hsr; widely used and extensively characterized cell line; listed in the CCLE (ref 18232)

Reference(s):

14858, 14947, 18232, 41750

Biosafety level:

1

Permissions and restrictions:

Morphology:

adherent, round to fusiform with polar neuritic processes, growing in mono- and multilayers; image; image

Medium:

90% RPMI 1640 + 10% h.i. FBS

Subculture:

seed out at ca. 2-3 x 10⁶ cells/80 cm²; split confluent culture 1:3 to 1:10 every 3-6 days using trypsin/EDTA

Incubation:

at 37 °C with 5% CO₂

Doubling time:

ca. 30-40 hours

Harvest:

cell harvest of ca. 25-30 x 10⁶ cells/80 cm²

Storage:

frozen in serum-free freezing medium (Bambanker^TM)

Mycoplasma:

original contamination was eliminated with Baytril (enrofloxacin), then negative in DAPI, microbiological culture, RNA hybridization, and continously in PCR assays

Immunology:

To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de.

Authenticity:

STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.

Cytogenetics:

human near-diploid karyotype with 20% polyploidy - 46-47<2n>X, -7, -8, +16, +22, +2mar, del(1)(p34), t(2;7)(p12;q11), add(4)(p15.3), add(5)(p15), add(9)(q34), add(10)(q24), add(11)(q24), add(14)(q32), ins(17;2)(q21;p24 hsr)x2 - del(1p34) and 2p24 hsr are typical of late-stage neuroblastoma - resembles published karyotype

Viruses:

ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV +, SMRV -

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