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Antibody System

[Antibodysystem] Anti-Human IL11RA Antibody (SAA2023)

Cat-No. RHG84901

AntibodySystem는 Primary Antibody Biosimilar - Research Grade

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제품 설명

Anti-Human IL11RA Antibody (SAA2023)

제품 번호

RHG84901

제품 특징

Species reactivity

Human

Host species

Human

Isotype

IgG4, kappa

Clonality

Monoclonal

Target

IL-11RA, sIL-11R, Interleukin-11 receptor subunit alpha, sIL-11RA, IL11RA, IL-11R-alpha, IL-11R subunit alpha, IL-11 receptor subunit alpha, sIL11RA

Concentration

1 mg/ml

Endotoxin level

Please contact with the lab for this information.

Purity

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Accession

Q14626

Applications

ELISA, FCM, WB

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Clone ID

SAA2023

Data Image

Flow-cytometry
Flow-cytometry using anti-human IL11RA antibody.IL11RA Transfected CHO cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human IL11RA antibody monoclonal antibody (Catalog # RHG84901 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-human antibody (Catalog # PHB96441) and cells analysed on a NovoCyte Flow Cytometer.
Flow-cytometry
Flow-cytometry using anti-human IL11RA antibody.HeLa cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human IL11RA antibody monoclonal antibody (Catalog # RHG84901 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-human antibody (Catalog # PHB96441) and cells analysed on a NovoCyte Flow Cytometer.
Western Blot
Various lysates were subjected to SDS PAGE followed by western blot with IL11RA antibody (RHG84901) at 1 µg/ml.

Lane 1: IL11RA transfected HEK293 cell lysate
Lane 2: Non-transfected HEK293 cell lysate

Second Ab: Goat Anti-Human IgG H&L Polyclonal antibody, HRP (PHB96431) at 0.1 μg/mL.

Predict MW: 40 kDa
Observed MW: 40 kDa