(주)모아바이오

YEASEN

[YEASEN] Annexin V-FITC/PI Apoptosis Detection Kit

Cat-No. 40302ES

40302ES08 / size: 5T
40302ES20 / size: 20T
40203ES50 / size: 50T
40203ES60 / size: 100T

YEASEN은 생명과학 연구 및 진단을 위한 고품질의 연구용 시약과 기기를 제공하는 글로벌 바이오 기업입니다.

제품 설명

Annexin V-FITC/PI Apoptosis Detection Kit

제품 번호

40302ES08 / size: 5T

40302ES20 / size: 20T

40203ES50 / size: 50T

40203ES60 / size: 100T

제품 설명

Description

Annexin V-FITC/PI Apoptosis Detection Kit uses FITC-labeled Annexin V as a probe to detect early apoptosis of cells.

The detection principle is that in normal living cells, phosphotidylserine (PS) is located on the inner side of the cell membrane, but in early apoptotic cells, PS reverses from the inner side to the surface of the cell membrane and is exposed to the extracellular environment. Annexin V is a Ca²⁺ -dependent phospholipid binding protein with a molecular weight of 35-36 kDa. Annexin V is a Ca²⁺ -dependent phospholipid binding protein with a high affinity for PS and binds to the membranes of early-apoptotic cells via externally exposed phosphatidylserine.

In addition, Propidium Iodide (PI) is provided in this kit to distinguish between surviving early cells and necrotic or late apoptotic cells. PI is a kind of nucleic acid dye, which can not penetrate the intact cell membrane of normal cells or early apoptotic cells, but can penetrate the cell membrane of late apoptotic and necrotic cells and make the nucleus red. Therefore, when Annexin V was used in combination with PI, PI was excluded from living cells (Annexin V-/PI-) and early apoptotic cells (Annexin V+/PI-). The apoptotic cells and necrotic cells were double positive by FITC and PI binding staining (Annexin V+/PI+).

This kit can be used for flow cytometry and fluorescence microscopy.

Features

The reagents in this kit are provided in liquid form for easy use.
The kit can be used for a variety of purposes: flow cytometry, fluorescence microscopy for detection.
Under the fluorescence microscope, the color distinction is obvious.

Applications

This kit can be used for flow cytometry and fluorescence microscopy.
This kit can detect the early apoptosis of cells.
This kit can distinguish between late apoptotic and necrotic cells.

Specifications

Application	The verified application of the kit was to detect apoptotic cells by flow cytometry or immunofluorescence cytology.
Packing form	Bottle

Components

Component No.	Name	40302ES20 (20T)	40302ES50 (50T)	40302ES60 (100T)
40302-A	Annexin V-FITC	100 μL	250 μL	500 μL
40302-B	PI Staining Solution	200 μL	500 μL	1.0 mL
40302-C	1×Binding Buffer	10 mL	25 mL	50 mL