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제품 설명
T7 High Yield RNA Synthesis Kit
제품 번호
10623ES10 / size: 10T
10623ES50 / size: 50T
10623ES60 / size: 100T
제품 설명
T7 High Yield RNA Synthesis Kit optimizes the transcription reaction system. The kit can synthesize the single-stranded RNA efficiently by using T7 RNA polymerase, the linear double-stranded DNA with the T7 promoter sequence as the template, NTPs as the substrate to control the DNA sequence downstream of the promoter. During transcription, modified nucleotides can be added to the substrate to prepare biotin or dye-labeled RNA.
This kit can synthesize long transcripts and short transcripts, RNA can be produced 100-200 μg with 1 μg of DNA template input. The RNA synthesized by transcription can be used for various downstream applications, such as RNA structure and function research, RNase protection, probe hybridization, RNAi, microinjection, and in vitro translation.
Figure 1: In vitro RNA transcription process
Feature
Application
| Components No. | Name | 10623ES50 (50 T) | 10623ES60 (100 T) | 10623ES70 (500 T) |
| 10623-A | T7 RNA Polymerase Mix | 100 μL | 200 μL | 1 mL |
| 10623-B | 10×Transcription Buffer | 100 μL | 200 μL | 1 mL |
| 10623-C | ATP (100mM) | 100 μL | 200 μL | 1 mL |
| 10623-D | CTP (100mM) | 100 μL | 200 μL | 1 mL |
| 10623-E | GTP (100mM) | 100 μL | 200 μL | 1 mL |
| 10623-F | UTP (100mM) | 100 μL | 200 μL | 1 mL |
| 10623-G | Control DNA Template (500ng/μL) | 10 μL | 20 μL | 100 μL |
Dry ice transportation. Store at -15℃ ~ -25℃, valid for two years.
Figure 1. Standard RNA was synthesized in vitro using T7 RNA synthesis kit.
The reaction was incubated in a PCR instrument at 37℃ for 2h and then purified by magnetic beads (Cat#12602). The yield result was analyzed by NanoDrop spectrophotometer as shown in Figure 1.
Figures 2. The transcription demonstration of different lengths of RNA by T7 kit respectively in the electrophoretogram (Figures 2A), the capillary electrophoresis diagram (Figures 2B), and the chromatogram (Figures 2C)
Figure 3. Synthesis of capped RNA in vitro.
The reaction was incubated in PCR instrument at 37℃ for 2h, and then purified by magnetic beads (Cat#12602). The yield result was assayed by NanoDrop spectrophotometer as shown in Figure 3A. The integrity result was analyzed by capillary electrophoresis as shown in Figure 3B.
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