(주)모아바이오

YEASEN

[YEASEN] Hieff Clone™ Zero TA Cloning Kit

Cat-No. 10907ES

10907ES08 / size: 5T
10907ES20 / size: 20T

YEASEN은 생명과학 연구 및 진단을 위한 고품질의 연구용 시약과 기기를 제공하는 글로벌 바이오 기업입니다.

제품 설명

Hieff Clone™ Zero TA Cloning Kit

제품 번호

10907ES08 / size: 5T

10907ES20 / size: 20T

제품 설명

Description

DH5α Fast Chemically Competent Cell from YEASEN is a competeny cell obtained by a special process of DH5α strain. It can be used for the chemical transformation of DNA with the conversion efficiency up to 10⁸cfu/μg plasmid DNA. It is widely used for efficient DNA cloning and plasmid amplification, and can ensure stable inheritance of high-copy plasmids. At the same time, it can be used to construt clones, blue-white spot screening. Deletion of recA1 and endA1 genes in DH5α cells ensured the stability of cloned DNA. F-DH5α competent cells were prepared by special technology, without heat shock at 42℃ or incubated at 37℃, only 10 minutes to complete a transformation.

DH5α competent cell genotypes: F- φ80 lac ZΔM15 Δ(lacZYA-arg F) U169 endA1 recA1 hsdR17(r_k-,m_k+) supE44λ- thi -1 gyrA96 relA1phoA

Features

High transformation efficiency: ≥1×10⁸ cfu/μg pUC19 DNA
Genetic markers that allow for blue/white colony screening
Rapid: It only takes 10 minutes to complete one transformation

Applications

Efficient DNA cloning derived from PCR, cDNA-generation reactions
recA1 marker facilitates working with difficult to transform DNA

Specifications

Species	Escherichia coil
Cell type	Chemical competent cell
Whether it contains an F’ episome	No
Efficiency	≥1×10⁸
Blue/white colony screening	Yes
Bacterial or yeast strains	DH5α
Amplification of the ccdB-containing vector	It is not suitable for amplification of ccdB-containing vectors