AFG Scientific은 2만 가지 이상 다양한 종류의 ELISA KITS와 Antibodies 제품들을 

High Quality 등급으로 전문 생산하며 합리적인 가격을 제공하는 미국의 Biological 제조사입니다

제품 설명

Bovine Cortisol (CORT) Elisa Kit

제품 번호

EK760226

제품 특징

시약 준비 과정

1. Prepare wash buffer
   1. Combine 20 ml concentrated wash buffer (whole bottle) with 580 ml DI water
2. Standard Dilute the standard pipette 50 ul standard diluent in each tube. Pipette 100 ul standard in the fifth tube. And take out 100 ul from the fifth five tube into the fourth. Pipette 50 ul from the fourth tube to the third tube and produce dilution series as below. The undiluted Standard serves as the high standard. Sample Diluent serves as the zero standard blank well.

Tube	6	5	4	3	2	1	0
ng/ml	135	90	60	30	15	7.5	0

결과 값 계산

Take the standard concentration as the horizontal, the OD value for the vertical, draw the standard curve on graph paper, find out the corresponding concentration according to the sample OD value by the sample curve, multiplied by the dilution multiple, or calculate the straight line regression equation of the standard curve with the standard concentration and the OD value, with the sample OD value in the equation, calculate the sample concentration, multiplied by the dilution factor, the result is the sample actual concentration. 

Test effectiveness: the average value of positive control ≥1.00; the average value of negative control ≤0.10. The critical value (CUT OFF) calculation: critical value = the average value of negative control + 0.15

Negative result : if the OD value 

Positive result: if the OD value ≥ CUT OFF, the sample is positive. 

Graphical Representation as follows

AFG Scientifics에서 다양한 제품을 찾아보세요! 

• Antibodies
• Chemicals
• Elisa Kits
• Chromatography

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