1. Microplate reader (wavelength: 450nm)
2. 37°C incubator (CO2 incubator for cell culture is not recommenced.)
3. Automated plate washer or multi-channel pipette/5ml pipettor (for manual washing purpose)
4. Precision single (0.5-10μL, 5-50μL, 20-200μL, 200-1000μL) and multi-channel pipette with disposable tips(Calibration is required before use.)
5. Sterile tubes and Eppendorf tubes with disposable tips
6. Absorbent paper and loading slot
7. Deionized or distilled water

This product is detected by QC department and meets performance required in the manual. (Laboratory Humidity: 20%-60%; Temperature: 18°C -25°C; Equilibrate TMB substrate to 37°C before staining. After adding into the ELISA wells, incubate for 15min at 37°C in dark.)

Due to different assay environments and operations, assay data below and standard curve are provided for reference. Experimenters should establish standard curve according to their own assay.

STD.(ng/ml)	OD-1	OD-2	Average
0	0.091	0.093	0.092
3.125	0.172	0.178	0.175
6.25	0.226	0.232	0.229
12.5	0.348	0.358	0.353
25	0.501	0.515	0.508
50	0.862	0.886	0.874
100	1.627	1.675	1.651
200	2.199	2.263	2.231

Add a certain amount of Anti-MPV into the sample. Calculate the recovery by comparing the measured value with the expected amount of Anti-MPV in the sample.

Dilute the sample with a certain amount of Anti-MPV at 1:2, 1:4 and 1:8 to get the recovery range.

Intra-assay Precision: samples with low, medium and high concentration are tested 20 times on the same plate.

Inter-assay Precision: samples with low, medium and high concentration are tested 20 times on three different plates.

Perform the stability test for the sealed kit at 37°C and 2-8°C and get relevant data.