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제품 설명

Chicken serum amyloid A (SAA) Elisa Kit

제품 번호

EK780116

제품 특징

시약 준비 과정

1. Prepare wash buffer
   1. Combine 20 ml concentrated wash buffer (whole bottle) with 580 ml DI water
2. Standard Dilute the standard pipette 50 ul standard diluent in each tube. Pipette 100 ul standard in the fifth tube. And take out 100 ul from the fifth five tube into the fourth. Pipette 50 ul from the fourth tube to the third tube and produce dilution series as below. The undiluted Standard serves as the high standard. Sample Diluent serves as the zero standard blank well.

Tube	6	5	4	3	2	1	0
ng/ml	45	30	20	10	5	2.5	0

결과 값 계산

Take the standard concentration as the horizontal, the OD value for the vertical, draw the standard curve on graph paper, find out the corresponding concentration according to the sample OD value by the sample curve, multiplied by the dilution multiple, or calculate the straight line regression equation of the standard curve with the standard concentration and the OD value, with the sample OD value in the equation, calculate the sample concentration, multiplied by the dilution factor, the result is the sample actual concentration. Test effectiveness: the average value of positive control ≥1.00; the average value of negative control ≤0.10. The critical value (CUT OFF) calculation: critical value = the average value of negative control + 0.15

Negative result : if the OD value 

Positive result: if the OD value ≥ CUT OFF, the sample is positive. 

Graphical Representation as follows 

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• Antibodies
• Chemicals
• Elisa Kits
• Chromatography

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