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Antibody System

[Antibodysystem] Romiplostim ELISA Kit

Cat-No. KGD76501

AntibodySystem는 Primary Antibody Biosimilar - Research Grade

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제품 설명

Romiplostim ELISA Kit

제품 번호

KGD76501

제품 특징

Catalog No.

KGD76501

Description

PRINCIPLE OF THE ASSAY This assay employs the quantitative sandwich enzyme immunoassay technique. An antibody specific for Romiplostim has been pre-coated onto a microplate. Standards or samples are pipetted into the wells and any Romiplostim present is bound by the immobilized antibody. After washing away any unbound substances, a biotin-labeled antibody specific for Romiplostim is added to the wells. After washing away any unbound substances, Streptavidin-HRP is added to the wells. Following a wash to remove any unbound enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of Romiplostim bound in the initial step. The color development is stopped and the intensity of the color is measured.

Applications

Used for the quantitative determination of Romiplostim concentration in serum and plasma.

Detection method

Colorimetric

Sample type

Plasma, Serum

Assay type

Quantitative

Range

11.72 - 750 pg/mL

Sensitivity

11.31 pg/mL

Precision

Intra-Assay Precision (Precision within an assay): <10%

Three samples of known concentration were tested sixteen times on one plate to assess intra-assay precision.

Inter-Assay Precision (Precision between assays): <15%

Three samples of known concentration were tested in twenty four separate assays to assess inter-assay precision.

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/mL)	355.1	74.7	17.5	350.0	79.3	23.1
Standard deviation	13.4	5.8	1.4	11.8	4.1	3.2
CV (%)	3.8	7.8	8.2	3.4	5.1	13.9

Recovery

80-120%

Shipping

2-8 ℃

Stability and Storage

When the kit was stored at the recommended temperature for 12 months, the signal intensity decreased by less than 20%. For unopened kits, if you want to prolong the storage time, please store the Standard, Detection A, Detection B and Microplate at - 20 ℃, the rest reagents should be store at 4℃.

Alternative Names

CAS: 267639-76-9, romiplostim (Nplate)

Background

Romiplostimis a fusion protein thrombopoietin (TPO) peptide analog that increases platelet counts by binding to and activating the human TPO receptor. Used to treat thrombocytopenia associated with chronic immune thrombocytopenia (ITP).

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