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제품 설명
Hieff Trans™ UltraAAV Transfection Reagent (PEI)
제품 번호
40823ES
제품 설명
This product is a linearized polyethyleneimine (Polyethylenimine Linear, PEI) specially developed for the large-scale production of AAV in suspension culture systems. It is a chemically synthesized product, free of animal-derived components, with low cytotoxicity. The product is provided in sterile liquid form and has a wide applicability for various AAV serotypes. It features strong DNA loading capacity, low transfection complex ratio, and high complex stability, facilitating large-scale AAV production.
Doubled Full Capsid Ratio
Greater Transfection Efficiency: Delivers 50% higher efficiency compared to top PEI.
Half input volume for equivalent transfection efficiency.
Lower cost
Production of AAV Viral Vectors
Protein production
Form | liquid |
Properties | Polyethylenimine Linear (PEI) |
Virus Type | AAV |
Components No. | Name | 40823ES01 | 40823ES03 | 40823ES10 | 40823ES60 |
40823 | Hieff Trans™ UltraAAV Transfection Reagent |
Hieff Trans™ UltraAAV Transfection Reagent products should be stored at 2-8℃ for 2 year.
Figure 1. Hieff Trans™ UltraAAV showing low inputs. The AAV9 was produced in suspension 293F cells with different input of Hieff Trans PEI AAV (Left, Plasmids input: 0.5 μg) or Plasmids (Right, Hieff trans PEI input 0.6 μL) dosages per million cells. The virus was harvested 72 hours post-transfection.
Figure 2. When UltraAAV and Fector VIR transfection reagents were used simultaneously for AAV9 virus packaging, UltraAAV demonstrated significantly higher viral full-capsid rates (tested on viral stock) at DNA-to-PEI ratios of 1:0.5 and 1:1. Notably, when the UltraAAV reagent dosage was halved, full-capsid rate were significantly superior to Fector VIR.
Figure 3. Hieff Trans™ UltraAAV showing ultra trans-effciency compared with top PEIs. AAV2, AAV5, AAV8, and AAV9 were produced in suspension 293F cells, with a DNA dosage of 1 µg per million cells. The virus was harvested 72 hours post-transfection, and the viral supernatant was analyzed.
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