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[Aviva] KLK3 ELISA Kit (Human)

Cat-No. OKEH00249

Aviva Systems Biology는 기초 연구부터 전임상, 신약 개발, 진단 워크플로우 전반에서 사용할 수 있도록

폴리클로널·모노클로날 항체, 재조합 단백질, ELISA 키트, 그리고 맞춤형 단백질·항체 개발 서비스를 제공합니다.

제품 설명

KLK3 ELISA Kit (Human)

제품 번호

OKEH00249

제품 특징

Product Info

Predicted Species Reactivity

Homo sapiens|Human

Application

ELISA-Sandwich

ELISA Kit Detection Method

Colorimetric, OD450 nm

ELISA Kit Duration

~ 3 Hours

ELISA Kit Principle

Aviva Systems Biology KLK3 ELISA Kit (Human) (OKEH00249) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for KLK3 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. After washing,a biotinylated detector antibody specific for KLK3 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample KLK3 captured in well.

ELISA Kit Range

0.156-10ng/mL

ELISA Kit Recovery

Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 103%

ELISA Kit Reproducibility

Mean Intra-assay CV%: < 5.5% (n = 20)
Mean Inter-assay CV%: < 10.2% (n = 20)

ELISA Kit Component

Component	Amount
KLK3 Microplate	96 Wells (12 x 8 Well strips)
KLK3 Lyophilized Standard	2
Sample Diluent	1 x 20 mL
100X Biotinylated KLK3 Detector Antibody	1 x 120 uL
100X Avidin-HRP Conjugate	1 x 120 uL
Detector Antibody Diluent	1 x 12 mL
Conjugate Diluent	1 x 12 mL
25X Wash Buffer	1 x 30 mL
TMB Substrate	1 x 10 mL
Stop Solution	1 x 10 mL

Reconstitution and Storage

Store as indicated in product manual.

Sample Type

Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids

Sensitivity

0.078 ng/mL

Specificity

Natural and recombinant Human Prostate-specific antigen

Assay Info

Assay Methodology: Quantitative Sandwich ELISA

Target Info

Gene Symbol	KLK3
Gene Full Name	kallikrein related peptidase 3
Alias Symbols	APS, gamma-seminoprotein, hK3, kallikrein-3, KLK2A1, P-30 antigen, prostate-specific antigen, PSA, semenogelase, seminin.
NCBI Gene Id	354
Protein Name	Prostate-specific antigen
Description of Target	Hydrolyzes semenogelin-1 thus leading to the liquefaction of the seminal coagulum.
Uniprot ID	P07288
Protein Accession #	NP_001025218.1
Nucleotide Accession #	NM_001030047.1
Protein Size (# AA)	261
Molecular Weight	29 kDa