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[Aviva] VEGFA ELISA Kit (Human)

Cat-No. OKAA00029-96W

Aviva Systems Biology는 기초 연구부터 전임상, 신약 개발, 진단 워크플로우 전반에서 사용할 수 있도록

폴리클로널·모노클로날 항체, 재조합 단백질, ELISA 키트, 그리고 맞춤형 단백질·항체 개발 서비스를 제공합니다.

제품 설명

Albumin ELISA Kit (Pig)

제품 번호

OKAA00029-96W

제품 특징

Product Info

Predicted Species Reactivity

Human

Application

ELISA

ELISA Kit Detection Method

Colorimetric, OD450 nm

ELISA Kit Reproducibility

The coefficient of variation of both intra-assay and inter-assay were less than 10%.

ELISA Kit Component

Components
2 vials human VEGF Standard lyophilized, 2000 pg/ml upon reconstitution
2 vials concentrated Biotin-Conjugate anti-human VEGF antibody
2 vials Streptavidin-HRP solution
1 bottle Standard /sample Diluent
1 bottle Biotin-Conjugate antibody Diluent
1 bottle Streptavidin-HRP Diluent
1 bottle Wash Buffer Concentrate 20x (PBS with 1% Tween-20)
1 vial Substrate Solution
1 vial Stop Solution
2 vials human FGF-basic Standard lyophilized, 500 pg/ml upon reconstitution
2 vials concentrated Biotin-Conjugate anti-human FGF-basic antibody

Additional Information

Sample Type: cell culture supernates, serum, and plasma

Reagents: REAGENTS
1. Aluminum pouches with a Microwell Plate coated with monoclonal antibody to human VEGF (8x12)
2. 2 vials human VEGF Standard lyophilized, 2000 pg/ml upon reconstitution
3. 2 vials concentrated Biotin-Conjugate anti-human VEGF polyclonal antibody
4. 2 vials Streptavidin-HRP solution,
5. 1 bottle Standard /sample Diluent
6. 1 bottle Biotin-Conjugate antibody Diluent
7. 1 bottle Streptavidin-HRP Diluent
8. 1 bottle Wash Buffer Concentrate 20x (PBS with 1% Tween-20)
9. 1 vial Substrate Solution
10. 1 vial Stop Solution
11. 3 pieces Adhesive Films
12. package insert

Sample Type

cell culture, supernates, serum, and plasma

Sensitivity

7 pg/ml

Predicted Homology Based on Immunogen Sequence

Human

Specificity

This assay recognizes both natural and recombinant human TSLP. The factors listed below were prepared at 50ng/ml in Standard /sample Diluent and assayed for cross-reactivity and no significant cross-reactivity or interference was observed.
Recombinant human: IL-1a, IL-1b, IL-2, IL-3, IL-4, IL-5, IL-6 ,
Recombinant mouse: IL-1a, IL-1b, IL-2, IL-4, IL-5, IL-6 ,
Recombinant rat: IL-1a , IL-1b, IL-2, IL-4, IL-6, IL-10, Leptin ,

Assay Info

Assay Method: Quantitative Sandwich ELISA

Target Info

Gene Symbol	VEGFA
Gene Full Name	vascular endothelial growth factor A
Alias Symbols	VPF, VEGF, MVCD1
NCBI Gene Id	7422
Protein Name	Vascular endothelial growth factor A
Description of Target	This gene is a member of the PDGF/VEGF growth factor family. It encodes a heparin-binding protein, which exists as a disulfide-linked homodimer. This growth factor induces proliferation and migration of vascular endothelial cells, and is essential for both physiological and pathological angiogenesis. Disruption of this gene in mice resulted in abnormal embryonic blood vessel formation. This gene is upregulated in many known tumors and its expression is correlated with tumor stage and progression. Elevated levels of this protein are found in patients with POEMS syndrome, also known as Crow-Fukase syndrome. Allelic variants of this gene have been associated with microvascular complications of diabetes 1 (MVCD1) and atherosclerosis. Alternatively spliced transcript variants encoding different isoforms have been described. There is also evidence for alternative translation initiation from upstream non-AUG (CUG) codons resulting in additional isoforms. A recent study showed that a C-terminally extended isoform is produced by use of an alternative in-frame translation termination codon via a stop codon readthrough mechanism, and that this isoform is antiangiogenic. Expression of some isoforms derived from the AUG start codon is regulated by a small upstream open reading frame, which is located within an internal ribosome entry site.
Uniprot ID	P15692
Protein Accession #	NP_001020537.2
Nucleotide Accession #	NM_001025366.2