General Description

Enzyme-linked immunosorbent assay (ELISA) is a labeled immunoassay, which is a commonly used for analytical biochemistry testing. An ELISA kit is a quick, convenient and simple product to quantitatively and qualitatively detect a specific vitamin in a sample extract. At present, the ELISA method has been included in food standard regulations by many countries to detect vitamins in food, grains, and health products.

Test Principle

Competitive ELISA utilizes two specific antibodies, an enzyme-conjugated antibody and secondary antibody. Two antibodies compete with the protein of interest. The presence of a color change means that the test is negative because the enzyme-conjugated antibody bound the antigens.

Main Procedures Based on the competitive ELISA method, both enzyme-conjugate antibody and secondary antibody are added and incubated, followed by a buffer wash. After wash step, the substrate is added to produce a color change and detection of color change by an ELISA reader.

Product Advantages

Competition ELISA methods

Low %RSD of within-batch and between-batch precision

High sensitivity and recovery rate

Simple and easy operation

Meet the relevant regulations and limit of quantitation requirements