CUSABIO는 암, 세포 생물학, 면역학, 신경과학, 후생유전학 등 연구 분야의 글로벌 고객에게 60,000개 이상의 검증된 항체,
10,000개 이상의 재조합 단백질, 660개 이상의 사이토카인 및 수천 개의 ELISA 키트를 제공하는 검증된 제조업체 입니다.
제품 설명
Chicken Immunoglobulin of Yolk,IgY ELISA Kit
제품 번호
CSB-E11635Ch
제품 특징
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of chicken IgY in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
| To assess the linearity of the assay, samples were spiked with high concentrations of chicken IgY in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:500000 | Average % | 89 | ||||
| Range % | 81-100 | |||||
| 1:1000000 | Average % | 96 | ||||
| Range % | 87-103 | |||||
| 1:2000000 | Average % | 97 | ||||
| Range % | 92-104 | |||||
| 1:4000000 | Average % | 92 | ||||
| Range % | 86-98 | |||||
| The recovery of chicken IgY spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 95 | 89-105 | ||||
| EDTA plasma (n=4) | 97 | 90-100 | ||||
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
| ng/ml | OD1 | OD2 | Average | Corrected |
| 5 | 2.781 | 2.911 | 2.846 | 2.737 |
| 2.5 | 2.316 | 2.342 | 2.329 | 2.220 |
| 1.25 | 1.579 | 1.584 | 1.582 | 1.473 |
| 0.625 | 0.901 | 0.883 | 0.892 | 0.783 |
| 0.312 | 0.443 | 0.436 | 0.440 | 0.331 |
| 0.156 | 0.315 | 0.319 | 0.317 | 0.208 |
| 0.078 | 0.249 | 0.247 | 0.248 | 0.139 |
| 0 | 0.108 | 0.110 | 0.109 |
The Chicken Immunoglobulin of Yolk (IgY) ELISA Kit is a highly sensitive and reliable tool for detecting IgY in chicken serum, plasma, and egg yolk samples. Developed using cutting-edge technology, this ELISA kit delivers accurate results with a detection range of 0.078 ng/mL-5 ng/mL and a sensitivity of 0.02 ng/mL.
This kit offers a quick and convenient assay time of 1-5 hours, with a sample volume of 50-100ul. The detection wavelength is 450 nm, making it ideal for immunology research applications. The assay principle is quantitative and uses a sandwich method of measurement, ensur...Read more
Cusabio<
MSDS
온라인 견적