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Cusabio

[Cusabio] Rat B-cell CLL/lymphoma 2 (BCL2) ELISA kit

Cat-No. CSB-E08854r


CUSABIO는 암, 세포 생물학, 면역학, 신경과학, 후생유전학 등 연구 분야의 글로벌 고객에게 60,000개 이상의 검증된 항체, 

10,000개 이상의 재조합 단백질, 660개 이상의 사이토카인 및 수천 개의 ELISA 키트를 제공하는 검증된 제조업체 입니다. 




제품 설명


Rat B-cell CLL/lymphoma 2 (BCL2) ELISA kit




제품 번호


CSB-E08854r




제품 특징


Product Details

Target Name
B-cell CLL/lymphoma 2
Alternative Names
Bcl2; Bcl-2; Apoptosis regulator Bcl-2
Abbreviation
Uniprot No.
Species
Rattus norvegicus (Rat)
Sample Types
serum, plasma, tissue homogenates
Detection Range
0.312 ng/mL-20 ng/mL
Sensitivity
0.078 ng/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Cell Biology
Assay Principle
quantitative
Measurement
Sandwich


Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.


Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rat BCL2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.


Sample
Serum(n=4) 
1:1Average %87
Range %80-95
1:2Average %95
Range %85-99
1:4Average %95
Range %86-102
1:8Average %98
Range %82-104


Recovery

The recovery of rat BCL2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample TypeAverage % RecoveryRange
Serum (n=5)9585-100
EDTA plasma (n=4)9587-102


Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.


ng/mlOD1OD2AverageCorrected
202.7452.7682.7572.572
102.1762.1652.1711.986
51.4851.4631.4741.289
2.50.8960.8640.8800.695
1.250.5690.5430.5560.371
0.6250.4250.4390.4320.247
0.3120.2960.2840.2900.105
00.1850.1840.185



ELISA Data Analysis