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Cusabio

[Cusabio] Human Homocysteine(HCY)ELISA Kit

Cat-No. CSB-E13814h


CUSABIO는 암, 세포 생물학, 면역학, 신경과학, 후생유전학 등 연구 분야의 글로벌 고객에게 60,000개 이상의 검증된 항체, 

10,000개 이상의 재조합 단백질, 660개 이상의 사이토카인 및 수천 개의 ELISA 키트를 제공하는 검증된 제조업체 입니다. 



제품 설명


Human Homocysteine(HCY)ELISA Kit



제품 번호


CSB-E13814h



제품 특징


Product Details

Target Name
Homocysteine(HCY)
Alternative Names
N/A
Abbreviation
Hcy
Species
Homo sapiens (Human)
Sample Types
serum, plasma, urine, tissue homogenates
Detection Range
0.78 nmol/mL-50 nmol/mL
Sensitivity
0.195 nmol/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Metabolism
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human HCY in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
　SampleSerum(n=4)
1:1Average %85
Range %80-90
1:2Average %98
Range %91-105
1:4Average %89
Range %84-96
1:8Average %93
Range %86-98
Recovery
The recovery of human HCY spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5)9691-101
EDTA plasma (n=4)9289-94
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
nmol/mlOD1OD2AverageCorrected
502.0822.1562.1191.997
251.4561.4891.4731.351
12.50.9410.9680.9550.833
6.250.5830.5970.5900.468
3.1250.3420.3670.3550.233
1.560.2480.2610.2550.133
0.780.1920.1980.1950.073
00.1210.1230.122　
Materials provided
  • A micro ELISA plate --- The 96-well plate has been pre-coated with an anti-human HCY antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
  • Two vials lyophilized standard ---Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
  • One vial Biotin-labeled HCY antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.
  • One vial HRP-avidin (100 x concentrate) (120 μl/bottle) ---Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
  • One vial Biotin-antibodyDiluent (15 ml/bottle) ---Dilute the Biotin-antibody.
  • One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution.
Materials not provided
  • A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
  • An incubator can provide stable incubation conditions up to 37°C±5°C.
  • Centrifuge
  • Vortex
  • Squirt bottle, manifold dispenser, or automated microplate washer
  • Absorbent paper for blotting the microtiter plate
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Human Hcy ELISA Kit was designed for the quantitative measurement of Human Hcy protein in serum, plasma, urine, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.78 nmol/mL-50 nmol/mL and the sensitivity is 0.195 nmol/mL .


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