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Geneaid

[Geneaid] GENEzol™ Reagent

Cat-No. GZR050, GZR100, GZR200


Geneaid는 분자 생물학 연구와 진단을 위한 고품질 핵산 추출 및 분석 솔루션을 제공하는 글로벌 생명과학 기업입니다.




제품 설명


GENEzol™ Reagent




제품 번호


GZR050, GZR100, GZR200




제품 특징


Introduction

RNA Extraction/Tri RNA

GENEzol™ Reagent is a phenol, chloroform and guanidine isothiocyanate based scalable solution for extracting high-quality total RNA as well as simultaneous extraction of RNA, DNA and protein from a wide variety of samples such as blood, buffy coat, plasma, serum, cultured cells and tissue. The extracted RNA can be used directly in a variety of downstream applications such as cDNA Library Construction, Cloning, RT-PCR (Endpoint), Real-Time PCR, Nuclease Protection Assays and Northern Blotting. GENEzol™ Reagent is a cost effective alternative for efficient total RNA extraction.

 

Description


Advantages (Cat. # GZR050, GZR100, GZR200)

  • Extract total RNA or simultaneous RNA, DNA and protein within 1 hour
  • Sample Volume: up to 300 μl of blood, buffy coat, serum, plasma, up to 5 x 106 cultured cells, 50-100 mg of tissue
  • A cost effective, scalable solution
  • Format: phenol, chlorform, guanidine isothiocyanate
  • Storage: dry at 2ºC to 25ºC




APPLICATIONS

cDNA Library Construction, Cloning, RT-PCR (Endpoint), Real-Time PCR, Nuclease Protection Assays, Northern Blotting




COMPONENTS

  • GENEzol™ Reagent




QUALITY CONTROL

GENEzol™ Reagent is tested on a lot-to-lot basis according to Geneaid’s ISO-certified quality management system. RNA from a 1 ml human blood sample is extracted using GENEzol™ Reagent. 10 µl from a 50 µl eluate of RNA is analyzed by electrophoresis on a 0.8% agarose gel.




GENEzol™ Reagent Comparison Data

Figure 1 .Bacterial RNA was extracted from 5 x 108 E. coli cells using our top competitor's TRI reagent and GENEzol™ Reagent. The E. coli cells were incubated with lysozyme for 5 minutes at room temperature. 1 ml of reagent was then added to the cell lysate. The RNA pellets were dissolved in 50 μl of RNase-free water.

TRI Reagent = 1, 2
GENEzol™ Reagent = 3, 4

 
Sample
ng/µl
260/280
260/230
Yield
1
241.3
1.77
0.66
12.1
2
260.2
1.83
0.85
13.0
3
315.6
1.78
0.75
15.8
4
293.4
1.81
0.80
14.7




GENEzol™ Reagent Functional Test Data

Figure 2 .RNA from a 1 ml human blood sample was extracted using GENEzol™ Reagent. 10 µl from a 50 µl eluate of RNA was analyzed by electrophoresis on a 0.8% agarose gel.

M = Geneaid 1 Kb DNA Ladder

 
Sample
RNA Conc.
260/280
Yield
1
119.9 µg/ml
1.82
6.0 µg
2
135.5 µg/ml
1.76
6.8 µg
3
176.1 µg/ml
1.77
8.8 µg




GENEzol™ Reagent real-time PCR Data

RNA was extracted from 200 µl of human blood (5.2 x 1010 copy/ml) using GENEzol™ TriRNA Pure Kit (red line).
RNA standard (107 copy/ml) (
light green line)
RNA standard (106 copy/ml) (
dark green line)
RNA standard (105 copy/ml) (
light blue line)
RNA standard (104 copy/ml) (
dark blue line)

Figure 3 .Quantitative analysis of human beta globin mRNA extracted by GENEzol™ using Taqman probe 1-step qRT-PCR assay. The assay was run on a BioRad IQ5 thermal cycler.



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